Transcriptome-wide identification of WRKY family genes and their expression profiling toward salicylic acid in Camellia japonica

被引：17

作者：

Yang, Xu ^{[1
]}

Zhou, Zhongcheng ^{[1
]}

Fu, Mingyue ^{[2
]}

Han, Muxian ^{[1
]}

Liu, Zhongbing ^{[3
]}

Zhu, Changye ^{[2
]}

Wang, Ling ^{[2
]}

Zheng, Jiarui ^{[2
]}

Liao, Yongling ^{[2
]}

Zhang, Weiwei ^{[2
]}

Ye, Jiabao ^{[2
]}

Xu, Feng ^{[2
]}

机构：

[1] Hubei Ecol Polytech Coll, Dept Forestry Ecol, Wuhan, Peoples R China

[2] Yangtze Univ, Coll Hort & Gardening, Nanhuan Rd 1, Jingzhou 434025, Hubei, Peoples R China

[3] Wuhan Univ Bioengn, Sch Hort & Landscape, Wuhan, Peoples R China

来源：

PLANT SIGNALING & BEHAVIOR | 2021年 / 16卷 / 01期

关键词：

Camellia japonica; WRKY; salicylic acid; transcription factor; stress; expression pattern; NEGATIVE REGULATORS; STRESS TOLERANCE; ABIOTIC STRESS; DEFENSE; ARABIDOPSIS; RICE; RESISTANCE; OVEREXPRESSION; SUPERFAMILY; UPSTREAM;

D O I：

10.1080/15592324.2020.1844508

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ornamental plant Camellia japonica is widely distributed worldwide and is susceptible to various environmental stresses. The WRKY transcription factor (TF) is an important node of plant tolerance. However, WRKY TFs from C. japonica have not been reported yet. In this study, 48 CjWRKYs, namely, CjWRKY1 to CjWRKY48, were identified. Protein structure analysis revealed that CjWRKY proteins contain a highly conserved motif (WRKYGQK) and two variant motifs (WRKYGKK and WRKYGRK). Phylogenetic analysis indicated that the 48 CjWRKYs can be divided into three groups, which are further classified into six subgroups, namely, I-C, II-a, II-b, II-c, II-e, and III, which contain 10, 6, 8, 13, 7, and 4 members, respectively. The expression patterns of 15 CjWRKYs under salicylic acid (SA) treatment were investigated by real-time quantitative PCR (qRT-PCR). Results showed that the 15 CjWRKYs could be induced by SA treatment. This study is the first to screen CjWRKYs and identify the expression profile of CjWRKYs under SA treatment and provides a theoretical basis for analyzing the function of CjWRKY genes to SA stress tolerance in C. japonica.

引用

页数：11

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