IDENTIFICATION OF EARLY DIAGNOSTIC ANTIGENS FROM SPIROMETRA ERINACEIEUROPAEI SPARGANUM SOLUBLE PROTEINS USING IMMUNOPROTEOMICS

被引：1

作者：

Hu, Dan Dan ^{[1
]}

Cui, Jing ^{[1
]}

Xiao, Di ^{[2
]}

Wang, Li ^{[1
]}

Liu, Li Na ^{[1
]}

Liu, Ruo Dan ^{[1
]}

Zhang, Jian Zhong ^{[2
]}

Wang, Zhong Quan ^{[1
]}

机构：

[1] Zhengzhou Univ, Coll Med, Dept Parasitol, Zhengzhou 450052, Peoples R China

[2] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Collaborat Innovat Ctr Diag & Treatment Infect Di, Beijing, Peoples R China

来源：

SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH | 2014年 / 45卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Spirometra erinaceieuropaei; early diagnostic antigen; immunoproteomics; plerocercoid (sparganum); soluble proteins; INTESTINAL EPITHELIAL-CELLS; SPIRALIS INFECTIVE LARVAE; TRICHINELLA-SPIRALIS; PROTEOMIC ANALYSIS; IN-VITRO; MANSONI; PURIFICATION; PROTEASE; CHINA;

D O I：

暂无

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

In order to identify early specific diagnostic antigens of Spirometra erinaceieuropaei (syn. S. erinacei or S. mansoni) sparganum, soluble proteins were analyzed by two-dimensional electrophoresis (2-DE) and western blotting probed with immune sera from infected mice at 14 days post-infection. From a total of approximately 462 proteins spots mainly distributed in pI range of 5-6.6 and with molecular mass of 25-48 kDa, 6 immuno-reactive protein spots with molecular mass of 31.8-38.3 kDa were characterized by MALDI-TOF/TOE-MS. Three proteins were identified as S. erinaceieuropaei cysteine protease, Toxoplasma gondii hypothetical protein and Pecten spp actin, while the remaining were unidentified. The cysteine protease from S. erinaceieuropaei soluble proteins recognized by early infection sera might be developed as diagnostic reagent for early detection of sparganosis.

引用

页码：576 / 583

页数：8