IDENTIFICATION OF EARLY DIAGNOSTIC ANTIGENS FROM SPIROMETRA ERINACEIEUROPAEI SPARGANUM SOLUBLE PROTEINS USING IMMUNOPROTEOMICS

被引：1
|
作者：
Hu, Dan Dan [1 ]
Cui, Jing [1 ]
Xiao, Di [2 ]
Wang, Li [1 ]
Liu, Li Na [1 ]
Liu, Ruo Dan [1 ]
Zhang, Jian Zhong [2 ]
Wang, Zhong Quan [1 ]
机构：
[1] Zhengzhou Univ, Coll Med, Dept Parasitol, Zhengzhou 450052, Peoples R China
[2] Chinese Ctr Dis Control & Prevent, Natl Inst Communicable Dis Control & Prevent, State Key Lab Infect Dis Prevent & Control, Collaborat Innovat Ctr Diag & Treatment Infect Di, Beijing, Peoples R China
来源：
SOUTHEAST ASIAN JOURNAL OF TROPICAL MEDICINE AND PUBLIC HEALTH | 2014年 / 45卷 / 03期
基金：
中国国家自然科学基金;
关键词：
Spirometra erinaceieuropaei; early diagnostic antigen; immunoproteomics; plerocercoid (sparganum); soluble proteins; INTESTINAL EPITHELIAL-CELLS; SPIRALIS INFECTIVE LARVAE; TRICHINELLA-SPIRALIS; PROTEOMIC ANALYSIS; IN-VITRO; MANSONI; PURIFICATION; PROTEASE; CHINA;
D O I：
暂无
中图分类号：
R1 [预防医学、卫生学];
学科分类号：
1004 ; 120402 ;
摘要：
In order to identify early specific diagnostic antigens of Spirometra erinaceieuropaei (syn. S. erinacei or S. mansoni) sparganum, soluble proteins were analyzed by two-dimensional electrophoresis (2-DE) and western blotting probed with immune sera from infected mice at 14 days post-infection. From a total of approximately 462 proteins spots mainly distributed in pI range of 5-6.6 and with molecular mass of 25-48 kDa, 6 immuno-reactive protein spots with molecular mass of 31.8-38.3 kDa were characterized by MALDI-TOF/TOE-MS. Three proteins were identified as S. erinaceieuropaei cysteine protease, Toxoplasma gondii hypothetical protein and Pecten spp actin, while the remaining were unidentified. The cysteine protease from S. erinaceieuropaei soluble proteins recognized by early infection sera might be developed as diagnostic reagent for early detection of sparganosis.
引用
收藏
页码：576 / 583
页数：8
相关论文
共 50 条
  • [1] Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid
    Lu, Yan
    Sun, Jia-Hui
    Lu, Li-Li
    Chen, Jia-Xu
    Song, Peng
    Ai, Lin
    Cai, Yu-Chun
    Li, Lan-Hua
    Chen, Shao-Hong
    KOREAN JOURNAL OF PARASITOLOGY, 2021, 59 (06): : 615 - 623
  • [2] A Retrieved Sparganum of Spirometra erinaceieuropaei from a Korean Man during Mechanical Thrombectomy
    Hwang, Yang-Ha
    Son, Wonsoo
    Kim, Yong-Won
    Kang, Dong-Hun
    Chang, Hyun-Ha
    Goo, Youn-Kyoung
    Hong, Yeonchul
    Chung, Dong-Il
    KOREAN JOURNAL OF PARASITOLOGY, 2020, 58 (03): : 309 - 313
  • [3] Levels of sparganum infections and phylogenetic analysis of the tapeworm Spirometra erinaceieuropaei sparganum in wild frogs from Henan Province in central China
    Wei, T.
    Zhang, X.
    Cui, J.
    Liu, L. N.
    Jiang, P.
    Wang, Z. Q.
    JOURNAL OF HELMINTHOLOGY, 2015, 89 (04) : 433 - 438
  • [4] Identification of early diagnostic antigens from major excretory-secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics
    Li Wang
    Jing Cui
    Dan Dan Hu
    Ruo Dan Liu
    Zhong Quan Wang
    Parasites & Vectors, 7
  • [5] Identification of early diagnostic antigens from major excretory-secretory proteins of Trichinella spiralis muscle larvae using immunoproteomics
    Wang, Li
    Cui, Jing
    Hu, Dan Dan
    Liu, Ruo Dan
    Wang, Zhong Quan
    PARASITES & VECTORS, 2014, 7
  • [6] Survey and genetic variation of Spirometra erinaceieuropaei sparganum in frogs and snakes from Guangxi of southern China
    Zhang, X.
    Cui, J.
    Wei, T.
    Li, L. Y.
    Jiang, J.
    Lu, J. C.
    Jiang, P.
    Liu, L. N.
    Wang, Z. Q.
    TROPICAL BIOMEDICINE, 2014, 31 (04) : 862 - 870
  • [7] Identification,structure and function of a novel tetraspaninhoniologue from Spirometra erinaceieuropaei
    Fu Qiongyao
    Lu Yajun
    Rao Langyu
    Chen Jinglong
    Wu Qiang
    Cai Qunfang
    Li Lihua
    Wu Lixian
    Lu Gang
    Asian Pacific Journal of Tropical Medicine, 2011, 4 (09) : 739 - 742
  • [8] Identification, structure and function of a novel tetraspaninhomologue from Spirometra erinaceieuropaei
    Fu Qiongyao
    Lu Yajun
    Rao Langyu
    Chen Jinglong
    Wu Qiang
    Cai Qunfang
    Li Lihua
    Wu Lixian
    Lu Gang
    ASIAN PACIFIC JOURNAL OF TROPICAL MEDICINE, 2011, 4 (09) : 739 - 742
  • [9] Immunoproteomic Analysis of the Excretory-Secretory Proteins from Spirometra mansoni Sparganum
    Hu, Dan Dan
    Cui, Jing
    Wang, Li
    Liu, Li Na
    Wei, Tong
    Wang, Zhong Quan
    IRANIAN JOURNAL OF PARASITOLOGY, 2013, 8 (03) : 408 - 416
  • [10] Identification of early diagnostic antigens in soluble proteins of Trichinella spiralis adult worms by Western blot
    Xu, D. M.
    Wen, H.
    Wang, L. A.
    Hu, C. X.
    Qi, X.
    Sun, G. G.
    Liu, R. D.
    Wang, Z. Q.
    Cui, J.
    TROPICAL BIOMEDICINE, 2017, 34 (01) : 191 - 198
12345