The Involvement of the Mid1/Cch1/Yvc1 Calcium Channels in Aspergillus fumigatus Virulence

被引:33
作者
de Castro, Patricia Alves [1 ]
Chiaratto, Jessica [1 ]
Winkelstroeter, Lizziane K. [1 ]
Pedro Bom, Vinicius Leite [1 ]
Zambelli Ramalho, Leandra Naira [2 ]
Goldman, Maria Helena S. [3 ]
Brown, Neil Andrew [1 ]
Goldman, Gustavo H. [1 ,4 ]
机构
[1] Univ Sao Paulo, Fac Ciencias Farmaceut Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil
[2] Univ Sao Paulo, Fac Med Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil
[3] Univ Sao Paulo, Fac Filosofia Ciencias & Letras Ribeirao Preto, BR-14049 Ribeirao Preto, Brazil
[4] Natl Lab Sci & Technol Bioethanol CTBE, Campinas, SP, Brazil
来源
PLOS ONE | 2014年 / 9卷 / 08期
基金
巴西圣保罗研究基金会;
关键词
HIGH-EFFICIENCY TRANSFORMATION; OXIDATIVE STRESS-RESPONSE; CELL-WALL INTEGRITY; SACCHAROMYCES-CEREVISIAE; CANDIDA-ALBICANS; FILAMENTOUS FUNGUS; CA2+ INFLUX; TRANSCRIPTION FACTORS; VACUOLAR MEMBRANE; NEUROSPORA-CRASSA;
D O I
10.1371/journal.pone.0103957
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Aspergillus fumigatus is a major opportunistic pathogen and allergen of mammals. Calcium homeostasis and signaling is essential for numerous biological processes and also influences A. fumigatus pathogenicity. The presented study characterized the function of the A. fumigatus homologues of three Saccharomyces cerevisiae calcium channels, voltage-gated Cch1, stretch-activated Mid1 and vacuolar Yvc1. The A. fumigatus calcium channels cchA, midA and yvcA were regulated at transcriptional level by increased calcium levels. The YvcA::GFP fusion protein localized to the vacuoles. Both Delta cchA and Delta midA mutant strains showed reduced radial growth rate in nutrient-poor minimal media. Interestingly, this growth defect in the Delta cchA strain was rescued by the exogenous addition of CaCl2. The Delta cchA, Delta midA, and Delta cchA Delta midA strains were also sensitive to the oxidative stress inducer, paraquat. Restriction of external Ca2+ through the addition of the Ca2+-chelator EGTA impacted upon the growth of the Delta cchA and Delta midA strains. All the A. fumigatus Delta cchA, Delta midA, and Delta yvcA strains demonstrated attenuated virulence in a neutropenic murine model of invasive pulmonary aspergillosis. Infection with the parental strain resulted in a 100% mortality rate at 15 days post-infection, while the mortality rate of the Delta cchA, Delta midA, and Delta yvcA strains after 15 days post-infection was only 25%. Collectively, this investigation strongly indicates that CchA, MidA, and YvcA play a role in A. fumigatus calcium homeostasis and virulence.
引用
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页数:12
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