Study of protein detection and ultrastructure of Brazilian rice starch during alkaline extraction

被引：35

作者：

Cardoso, Mateus B. ^{[1
]}

Sarnios, Dimitrios ^{[1
]}

Silveira, Nadya P. ^{[1
]}

机构：

[1] Univ Fed Rio Grande do Sul, Inst Quim, Lab Instrumentat & Dinam Mol, BR-91501970 Porto Alegre, RS, Brazil

来源：

STARCH-STARKE | 2006年 / 58卷 / 07期

关键词：

rice starch; alkaline extraction; protein detection; starch ultrastructure;

D O I：

10.1002/star.200600495

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Alkaline extraction of Brazilian rice starch was studied. UV/Vis and fluorescence spectroscopy were employed as an innovative way for protein detection during the extraction. Moreover, the results were compared to the traditional biuret colorimetric test. In parallel, the ultrastructure of starch was monitored by small angle X-ray scattering (SAXS) during every extraction step. A progressive reduction of the granules organization was observed, which was also detected by the loss of birefringence under polarized light. The external integrity of starch granules after complete removal of proteins was maintained, as verified by scanning electron microscopy (SEM). The size of the granules, determined by the Mie scattering method varied between 3 and 8 mu m.

引用

页码：345 / 352

页数：8

共 39 条

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