HPTLC Quantification of Some Flavonoids in Extracts of Satureja hortensis L. Obtained by Use of Different Techniques

A study of quantitative analysis (if some flavonoids in Satureia hortensis L. extracts is presented. The HPTLC analyses were performed in a normal chromatographic chamber on silica gel plates, with an appropriate mobile phase. Photodensitometric evaluation of the chromatographic plates was performed by UV-visible absorbance at 320 nm for rosmarinic acid and 349 nm for luteoline, after pulverizing with NTS reagent (diphenylboryloxyethylamine). Quantitative analyses of flavonoids were performed by use of calibration curves. The quantity of compounds extracted depends on the composition of the extraction solvent and on the extraction technique. The best results were obtained by heating under reflux with systems that contained 60-70% ethanol. Under these conditions it was found that this plant contained approximately 34 mg g(-1) rosmarinic acid and 30 mg g(-1) luteoline. The results obtained were compared and cot-related with extraction method and confirm this plant contains important amounts of flavonoids and phenolic acids.