Homology-Directed Repair in Zebrafish: Witchcraft and Wizardry?

被引：12

作者：

Prill, Kendal ^{[1
]}

Dawson, John F. ^{[1
]}

机构：

[1] Univ Guelph, Dept Mol & Cellular Biol, Guelph, ON, Canada

来源：

FRONTIERS IN MOLECULAR BIOSCIENCES | 2020年 / 7卷

关键词：

CRISPR; genome modification; homology directed repair; zebrafish; precision medicine and genomics; END RESECTION; GENE; EXPRESSION;

D O I：

10.3389/fmolb.2020.595474

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Introducing desired mutations into the genome of model organisms is a priority for all research focusing on protein function and disease modeling. The need to create stable mutant lines has resulted in the rapid advancement of genetic techniques over the last few decades from chemical mutagenesis and zinc finger nucleases to clustered regularly interspaced short palindromic repeats (CRISPR) and homology-directed repair (HDR). However, achieving consistently high success rates for direct mutagenesis in zebrafish remains one of the most sought-after techniques in the field. Several genes have been modified using HDR in zebrafish, but published success rates range widely, suggesting that an optimal protocol is required. In this review, we compare target genes, techniques, and protocols from 50 genes that were successfully modified in zebrafish using HDR to find the statistically best variables for efficient HDR rates.

引用

页数：9

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