The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species-and strain-dependent genome context variation

被引：164
作者：
Harris, AKP
Williamson, NR
Slater, H
Cox, A
Abbasi, S
Foulds, I
Simonsen, HT
Leeper, FJ
Salmond, GPC
机构：
[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England
[2] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England
来源：
MICROBIOLOGY-SGM | 2004年 / 150卷
关键词：
D O I：
10.1099/mic.0.27222-0
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprocligiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.
引用
收藏
页码：3547 / 3560
页数：14
相关论文
共 69 条
[1]   The crystal structure of 8-amino-7-oxononanoate synthase: A bacterial PLP-dependent, acyl-CoA-condensing enzyme [J].
Alexeev, D ;
Alexeeva, M ;
Baxter, RL ;
Campopiano, DJ ;
Webster, SP ;
Sawyer, L .
JOURNAL OF MOLECULAR BIOLOGY, 1998, 284 (02) :401-419
[2]   MOLECULAR CHARACTERIZATION OF THE SOXRS GENES OF ESCHERICHIA-COLI - 2 GENES CONTROL A SUPEROXIDE STRESS REGULON [J].
AMABILECUEVAS, CF ;
DEMPLE, B .
NUCLEIC ACIDS RESEARCH, 1991, 19 (16) :4479-4484
[3]   Antibiotic resistance and putative virulence factors of Serratia marcescens with respect to O and K serotypes [J].
Aucken, HM ;
Pitt, TL .
JOURNAL OF MEDICAL MICROBIOLOGY, 1998, 47 (12) :1105-1113
[4]  
BLAZEVIC DJ, 1970, GENUS SERRATIA
[5]   EXCRETION OF A PROTEASE BY SERRATIA-MARCESCENS [J].
BRAUN, V ;
SCHMITZ, G .
ARCHIVES OF MICROBIOLOGY, 1980, 124 (01) :55-61
[6]   ZntR is a Zn(II)-responsive MerR-like transcriptional regulator of zntA in Escherichia coli [J].
Brocklehurst, KR ;
Hobman, JL ;
Lawley, B ;
Blank, L ;
Marshall, SJ ;
Brown, NL ;
Morby, AP .
MOLECULAR MICROBIOLOGY, 1999, 31 (03) :893-902
[7]   THE ISOLATION AND CHARACTERIZATION OF (3R,5R)-CARBAPENAM-3-CARBOXYLIC AND (3S,5R)-CARBAPENAM-3-CARBOXYLIC ACID FROM SERRATIA AND ERWINIA SPECIES AND THEIR PUTATIVE BIOSYNTHETIC ROLE [J].
BYCROFT, BW ;
MASLEN, C ;
BOX, SJ ;
BROWN, AG ;
TYLER, JW .
JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1987, (21) :1623-1625
[8]   Prodigiosin induces apoptosis of B and T cells from B-cell chronic lymphocytic leukemia [J].
Campàs, C ;
Dalmau, M ;
Montaner, B ;
Barragán, M ;
Bellosillo, B ;
Colomer, D ;
Pons, G ;
Pérez-Tomás, R ;
Gil, J .
LEUKEMIA, 2003, 17 (04) :746-750
[9]   Clinical relevance and virulence factors of pigmented Serratia marcescens [J].
Carbonell, GV ;
Della Colleta, HHM ;
Yano, T ;
Darini, ALC ;
Levy, CE ;
Fonseca, BAL .
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2000, 28 (02) :143-149
[10]   Analysis of the prodiginine biosynthesis gene cluster of Streptomyces coelicolor A3(2):: new mechanisms for chain initiation and termination in modular multienzymes [J].
Cerdeño, AM ;
Bibb, MJ ;
Challis, GL .
CHEMISTRY & BIOLOGY, 2001, 8 (08) :817-829
1234567