D-Penicillamine and bovine serum albumin co-stabilized copper nanoclusters with remarkably enhanced fluorescence intensity and photostability for ultrasensitive detection of Ag+

被引:25
作者
Li Ruiyi [1 ]
Wang Huiying [1 ]
Zhou Xiaoyan [1 ]
Liao Xiaoqing [1 ]
Sun Xiulan [2 ]
Li Zaijun [1 ,3 ]
机构
[1] Jiangnan Univ, Sch Chem & Mat Engn, Wuxi 214122, Peoples R China
[2] Jiangnan Univ, Sch Food Sci & Technol, Wuxi 214122, Peoples R China
[3] Minist Educ, Key Lab Food Colloids & Biotechnol, Wuxi 214122, Peoples R China
基金
中国国家自然科学基金;
关键词
GOLD NANOCLUSTERS; GREEN SYNTHESIS; SCATTERING; SILVER(I); OXIDE; IONS; NANOPARTICLES; MERCURY(II); BIOSENSOR; PROBE;
D O I
10.1039/c5nj02615f
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Copper nanoclusters (CuNCs) have become promising nanomaterials due to their excellent electronic conductivity and biocompatibility. In this study, we reported a facile synthesis of D-penicillamine and bovine serum albumin co-stabilized copper nanoclusters (BSA/DPA-CuNCs) and the use as optical probe for the detection of Ag+. Herein, Cu2+ was reduced into Cu-0 by D-penicillamine, further leading to form DPA-CuNCs. Then, BSA was introduced into DPA-CuNCs to obtain BSA/DPA-CuNCs by their electrostatic attraction. The as-prepared BSA/DPA-CuNCs exhibit remarkably enhanced fluorescence intensity and photostability. The peak fluorescence intensity is more than 2-fold that of DPA-CuNCs. Due to the synergistic effect between DPA and BSA, the nanosensor based on BSA/DPA-CuNCs gives ultrasensitive fluorescence response towards Ag+. Its peak fluorescence intensity linearly decreases with the increase of Ag+ concentration in the range of 1.0 x 10(-8) to 1.0 x 10(-16) M with the detection limit of 4.3 x 10(-17) M (S/N = 3). The method provides the advantage of sensitivity, repeatability and stability. It has been successfully applied in the detection of Ag+ in water samples. The study also opens a new avenue for the fabrication of functional metal nanoclusters that hold great promise in potential applications such as nanosensors, biocatalysts and molecular carriers.
引用
收藏
页码:732 / 739
页数:8
相关论文
共 26 条
[1]   FLUORESCENCE QUENCHING STUDIES WITH PROTEINS [J].
EFTINK, MR ;
GHIRON, CA .
ANALYTICAL BIOCHEMISTRY, 1981, 114 (02) :199-227
[2]   A novel biosensor for copper(II) ions based on turn-on resonance light scattering of ssDNA templated silver nanoclusters [J].
Feng, Da-Qian ;
Liu, Guoliang ;
Wang, Wei .
JOURNAL OF MATERIALS CHEMISTRY B, 2015, 3 (10) :2083-2088
[3]   Blue-Emitting Copper Nanoclusters Synthesized in the Presence of Lysozyme as Candidates for Cell Labeling [J].
Ghosh, Rama ;
Sahoo, Amaresh Kumar ;
Ghosh, Siddhartha Sankar ;
Paul, Anumita ;
Chattopadhyay, Arun .
ACS APPLIED MATERIALS & INTERFACES, 2014, 6 (06) :3822-3828
[4]   Ag nanocluster-based label-free catalytic and molecular beacons for amplified biosensing [J].
Gong, Liang ;
Kuai, Hailan ;
Ren, Songlei ;
Zhao, Xu-Hua ;
Huan, Shuang-Yan ;
Zhang, Xiao-Bing ;
Tan, Weihong .
CHEMICAL COMMUNICATIONS, 2015, 51 (60) :12095-12098
[5]   Green synthesis of peptide-templated fluorescent copper nanoclusters for temperature sensing and cellular imaging [J].
Huang, Hong ;
Li, Hua ;
Wang, Ai-Jun ;
Zhong, Shu-Xian ;
Fang, Ke-Ming ;
Feng, Jiu-Ju .
ANALYST, 2014, 139 (24) :6536-6541
[6]   Fluorescein-5-isothiocyanate-conjugated protein-directed synthesis of gold nanoclusters for fluorescent ratiometric sensing of an enzyme-substrate system [J].
Ke, Chen-Yi ;
Wu, Yun-Tse ;
Tseng, Wei-Lung .
BIOSENSORS & BIOELECTRONICS, 2015, 69 :46-53
[7]   Multifunctional Au nanoclusters for targeted bioimaging and enhanced photodynamic inactivation of Staphylococcus aureus [J].
Khlebtsov, Boris ;
Tuchina, Elena ;
Tuchin, Valery ;
Khlebtsov, Nikolai .
RSC ADVANCES, 2015, 5 (76) :61639-61649
[8]   Ratiometric fluorescence detection of silver ions using thioflavin T-based organic/inorganic hybrid supraparticles [J].
Li, Yan-Yun ;
Zhang, Min ;
Lu, Ling-Fei ;
Zhu, Anwei ;
Xia, Fei ;
Zhou, Tianshu ;
Shi, Guoyue .
ANALYST, 2015, 140 (17) :6108-6113
[9]   Fast synthesis of copper nanoclusters through the use of hydrogen peroxide additive and their application for the fluorescence detection of Hg2+ in water samples [J].
Liao Xiaoqing ;
Li Ruiyi ;
Li Zaijun ;
Sun Xiulan ;
Wang Zhouping ;
Liu Junkang .
NEW JOURNAL OF CHEMISTRY, 2015, 39 (07) :5240-5248
[10]   Ultra sensitive and wide-range pH sensor based on the BSA-capped Cu nanoclusters fabricated by fast synthesis through the use of hydrogen peroxide additive [J].
Liao Xiaoqing ;
Li Ruiyi ;
Long Xiaohuan ;
Li Zaijun .
RSC ADVANCES, 2015, 5 (60) :48835-48841