The intermittent administration of ethanol during the juvenile period produces changes in the expression of hippocampal genes and proteins and deterioration of spatial memory

被引:15
作者
Contreras, Ana [1 ]
Morales, Lidia [1 ]
Del Olmo, Nuria [1 ]
机构
[1] Univ CEU San Pablo, Dept Ciencias Farmaceut & Salud, Fac Farm, Madrid, Spain
关键词
Adolescence; Intermittent ethanol drinking; Spatial memory; NMDA; AMPA; GSK3; beta; METABOTROPIC GLUTAMATE RECEPTORS; GLYCOGEN-SYNTHASE KINASE-3-BETA; LONG-TERM POTENTIATION; ALCOHOL EXPOSURE; BINGE DRINKING; WORKING-MEMORY; MESSENGER-RNA; SYNAPTIC PLASTICITY; DENTATE GYRUS; ADOLESCENT;
D O I
10.1016/j.bbr.2019.112033
中图分类号
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要
Background: Binge drinking is a pattern of alcohol intake characterized by excessive and intermittent alcohol consumption over a very short period of time that is more used during adolescence. We aim to compare the lasting effects of a chronic-moderate vs. this intermittent-excessive way of alcohol intake during adolescence in spatial memory and in the expression of glutamatergic receptors and GSK3 beta activity. Methods: Adolescent male Wistar rats were given ethanol/saline i.p. injections in four different groups: High-I (4 g/kg of a 25% (vol/vol) every 3 days), Low-I (1 g/kg of a 5% (vol/vol) every 3 days), M (0.3 g/kg of a 2.5% (vol/vol) daily) and Control (C, sterile isotonic saline daily). Rats received ethanol for up to five 3-day cycles. Spatial memory was measured by spontaneous alternation in the Y-Maze. Gene and protein expression of hippocampal proteins were also analysed. Results: Both high- and low-intermittent ethanol administration produced spatial memory impairment and changes in glutamatergic receptors gene expression were observed regardless of the pattern of exposure. High doses of intermittent alcohol administration produced an increase of phosphorylation of GSK3 beta Ser9. Moreover, moderate alcohol administration produced a down-regulation of the AMPAR 2/3 ratio despite lack of spatial memory deficits. Conclusions: Excessive and intermittent ethanol exposure during adolescence impaired the spatial memory processes during adulthood regardless of the amount of alcohol administered. Moreover, chronic-moderate and intermittent pattern induced changes in the expression of glutamatergic receptors. In addition, high-intermittent ethanol exposure during adolescence inactivated GSK3 beta by increasing its phosphorylation in Ser9.
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页数:9
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