Acetic acid effects on methanogens in the second stage of a two-stage anaerobic system

被引：21

作者：

Xiao, Keke ^{[1
,2
]}

Guo, Chenghong ^{[1
,2
]}

Zhou, Yan ^{[1
,2
]}

Maspolim, Yogananda ^{[1
,2
]}

Ng, Wun-Jern ^{[1
,2
]}

机构：

[1] Nanyang Technol Univ, Adv Environm Biotechnol Ctr, Nanyang Environm & Water Res Inst, Singapore 637141, Singapore

[2] Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore 639798, Singapore

来源：

CHEMOSPHERE | 2016年 / 144卷

基金：

新加坡国家研究基金会;

关键词：

Two-stage anaerobic system; Acetic acid inhibition; Acclimation; Hydrogenotrophic methanogens; Pyrosequencing; Biomass activity; ORGANIC LOADING RATE; RIBOSOMAL-RNA GENES; MICROBIAL COMMUNITY; SINGLE-STAGE; DIGESTION; SLUDGE; DEGRADATION; DYNAMICS; SOLIDS; FERMENTATION;

D O I：

10.1016/j.chemosphere.2015.10.035

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

This study reports on biomass tolerance towards high concentrations of acetic acid (HAc) within the system. Biomass from the second stage of a two-stage anaerobic sludge digestion system was used for this study. Microbial community analysis by 454 pyrosequencing highlighted hydrogenotrophic Methanomicrobiales was the predominant archaeal population in the second stage (>99% of the total archaeal community). Second stage biomass degraded HAc up to 4200 mg HAc L-1 without observable lag phase. However, at HAc-shock loading of 7400 mg HAc L-1, it showed a one day lag phase associated with decreased biomass activity. After stepwise HAc-acclimation over 27 d, the biomass degraded HAc of up to 8200 mg HAc L-1 without observable lag phase. The dominance of Methanomicrobiales had remained unchanged in proportion - while the total archaeal population increased during acclimation. This study showed stepwise acclimation could be an approach to accommodate HAc accumulation and hence higher concentrations resulting from an enhanced first stage. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：1498 / 1504

页数：7

共 31 条

[11] Evaluating a correlation between volatile suspended solid and adenosine 5′-triphosphate levels in anaerobic treatment of high organic suspended solids wastewater [J].