EVALUATION OF THE CYTOGENETIC STATUS OF HUMAN LYMPHOCYTES AFTER EXPOSURE TO A HIGH CONCENTRATION OF BEE VENOM IN VITRO

被引：37

作者：

Garaj-Vrhovac, Verica ^{[1
]}

Gajski, Goran ^{[1
]}

机构：

[1] Inst Med Res & Occupat Hlth, Mutagenesis Unit, HR-10001 Zagreb, Croatia

来源：

ARHIV ZA HIGIJENU RADA I TOKSIKOLOGIJU-ARCHIVES OF INDUSTRIAL HYGIENE AND TOXICOLOGY | 2009年 / 60卷 / 01期

关键词：

comet assay; DNA damage; in vitro studies; melittin; micronucleus test; BLOCK MICRONUCLEUS ASSAY; APIS-MELLIFERA VENOM; PERIPHERAL-BLOOD LYMPHOCYTES; GEL-ELECTROPHORESIS ASSAY; COMET ASSAY; DNA-DAMAGE; INDUCED APOPTOSIS; INDIVIDUAL CELLS; MELITTIN; VIVO;

D O I：

10.2478/10004-1254-60-2009-1896

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

Several studies have reported radioprotective, antimutagenic, anti-inflammatory, anti nociceptive, and anticancer effects of bee venom both in the cell and the whole organism. The aim of this study was to assess the effects of a single high dose of 100 mu g mL(-1) of whole bee venom in human lymphocytes in vitro over a variety of time spans (from 10 min to 24 h). After the treatment, we used the comet assay and micronucleus test to see the effect of bee venom on the cell. The cornet assay confirmed that the venom damaged the DNA molecule. Tail length, tail intensity, tail moment showed a significant increase (P<0.05). The percentage of long-tailed nuclei (LTN) with the tail length exceeding the 95(th) percentile also increased in a time-dependent manner. The micronucleus parameters (number of micronuclei, nucleoplasmic bridges, and nuclear buds) also showed a significant time-dependent increase (P<0.05). This research indicates that high concentrations of bee venom can lead to cellular instability. Further research is needed to understand the mechanism of action of bee venom and its components in human cells and to see if this natural product may find application in medicine.

引用

页码：27 / 34

页数：8

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