An external ion microbeam for studies of biological samples

被引:19
作者
Sakai, T [1 ]
Naitoh, Y
Kamiya, T
Matsuyama, S
Gotoh, K
Yotoka, S
Yamasaki, H
Ishii, K
机构
[1] Japan Atom Energy Res Inst, Takasaki Radiat Chem Res Estab, Takasaki, Gumma 3701292, Japan
[2] Tohoku Univ, Dept Quantum Sci & Energy Engn, Aoba Ku, Sendai, Miyagi 9808579, Japan
[3] Tohoku Univ, Fac Agr, Aoba Ku, Sendai, Miyagi 9818555, Japan
关键词
external beam; Kapton window : micro-PIXE; nuclear microprobe; biological samples; spatial resolution;
D O I
10.1007/BF02784193
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
A new external ion-beam system was developed and combined with a light ion-microbeam system in JAERI Takasaki. The system is designed for micro-particle-induced X-ray emission analysis of biological samples in air environment with 1 mu m spatial resolution. One of the most serious problems in keeping such a high spatial resolution is multiple scattering in a beam exit window. Thin Kapton film (7.5 mu m thick) was adopted as the exit window as well as a sample backing foil to minimize the distance between the film and samples. The Lifetime of the foil under ion irradiation and spatial resolution of the external microbeam were investigated. The results shows that the film can endure sufficient long-time irradiation to take elemental maps and the resolution can be kept nearly 1 mu m.
引用
收藏
页码:77 / 82
页数:6
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