Assignment of human elongation factor 1 alpha genes: EEF1A maps to chromosome 6q14 and EEF1A2 to 20q13.3

被引：77
作者：
Lund, A
Knudsen, SM
Vissing, H
Clark, B
Tommerup, N
机构：
[1] AARHUS UNIV,INST CHEM,DEPT BIOSTRUCT CHEM,DK-8000 AARHUS,DENMARK
[2] BISPEBJERG HOSP,DEPT CLIN BIOCHEM,DK-2400 COPENHAGEN,DENMARK
[3] NOVO NORDISK AS,MOL & CELLULAR BIOL,DK-2880 BAGSVAERD,DENMARK
[4] JOHN F KENNEDY INST,DANISH CTR HUMAN GENOME RES,DK-2600 GLOSTRUP,DENMARK
来源：
GENOMICS | 1996年 / 36卷 / 02期
关键词：
D O I：
10.1006/geno.1996.0475
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The human elongation factor 1 alpha gene family consists of at least 2 actively transcribed genes, EEF1A and EEF1A2, and more than 18 homologous loci. EEF1A2 is expressed in a tissue-specific manner, whereas EEF1A is expressed ubiquitously, and both of them can function in translation. An EEF1A cDNA probe has previously been shown to cross-hybridize with several human chromosomes, but the location of the functional gene has not been established. We have mapped the functional EEF1A gene to 6q14 by combined fluorescence in situ hybridization (FISH) and PCR analysis of a somatic cell hybrid panel and mapped EEF1A2 to 20q13.3 by FISH. In addition, the 11 strongest cross-hybridizing loci (EEF1AL2-EEF1AL13) were mapped by FISH to 12p12, 9q34, 7p15-p21, 19q13, 3q26-q27, 7q33-q35, 1p13-p22, 2q12-q14, 5p12-q11, 1q31-q32, and Xq21. (C) 1996 Academic Press, Inc.
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页码：359 / 361
页数：3
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