Identification of cattle carrying alleles associated with resistance and susceptibility to the Bovine Leukemia Virus progression by real-time PCR

被引：15
作者：
Forletti, A. [1 ]
Juliarena, M. A. [1 ,3 ]
Ceriani, C. [1 ,3 ]
Amadio, A. F. [2 ,3 ]
Esteban, E. [1 ]
Gutierrez, S. E. [1 ,3 ]
机构：
[1] FCV UNCPBA, Lab Virol, Ctr Invest Vet Tandil CIVETAN, CONICET,Dept SAMP, RA-7000 Buenos Aires, DF, Argentina
[2] EEA Rafaela, Inst Nacl Tecnol Agr, RA-2300 Rafaela, Santa Fe, Argentina
[3] CONICET Consejo Nacl Invest Cient & Tecn, Buenos Aires, DF, Argentina
来源：
RESEARCH IN VETERINARY SCIENCE | 2013年 / 95卷 / 03期
关键词：
BoLA DRB3; Bovine Leukemia Virus; Resistance; Susceptibility; PCR; Real-time PCR; BOLA-DRB3; ALLELES; GENETIC ASSOCIATION; HOLSTEIN CATTLE; DRB3; BOLA DRB3.2; DAIRY-COWS; MASTITIS; SEQUENCE; POLYMORPHISM; INFECTION;
D O I：
10.1016/j.rvsc.2013.07.018
中图分类号：
S85 [动物医学（兽医学）];
学科分类号：
0906 ;
摘要：
Previous studies have shown a significant association between polymorphisms of the BoLA DRB3 gene and Bovine Leukemia Virus (BLV) infection profile. The presence of allele *1501 has been associated with high proviral load in peripheral blood while allele *0902 has been associated with low proviral load. The purpose of this study was to develop allele-specific real-time PCRs to identify cattle carrying alleles associated with resistance (BoLA DRB3*0902) or susceptibility (BoLA DRB3*1501) to the BLV progression. Specific primers were designed and differential amplification was carried out by real-time PCR and monitored by SYBR (R) Green dye in DNA samples from peripheral blood. Conditions were also adjusted for traditional PCR amplification (end point amplification). These methods are rapid, simple and suitable for high throughput screening, and could aid in marker-assisted selection of BLV-resistant and susceptible cattle. (C) 2013 Elsevier Ltd. All rights reserved.
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收藏
页码：991 / 995
页数：5
相关论文
共 23 条
[1]   A rapid and robust sequence-based genotyping method for BoLA-DRB3 alleles in large numbers of heterozygous cattle [J].
Baxter, R. ;
Hastings, N. ;
Law, A. ;
Glass, E. J. .
ANIMAL GENETICS, 2008, 39 (05) :561-563
[2]   Allelic discrimination of genetic human prion diseases by real-time PCR genotyping [J].
Calero, Olga ;
Hortigueela, Rafael ;
Albo, Carmen ;
de Pedro-Cuesta, Jesus ;
Calero, Miguel .
PRION, 2009, 3 (03) :146-150
[3]   Bovine lymphocyte antigen class II alleles as risk factors for high somatic cell counts in milk of lactating dairy cows [J].
Dietz, AB ;
Cohen, ND ;
Timms, L ;
Kehrli, ME .
JOURNAL OF DAIRY SCIENCE, 1997, 80 (02) :406-412
[4]   Genetic association of bovine lymphocyte antigen DRB3 alleles with immunological traits of Holstein cattle [J].
Dietz, AB ;
Detilleux, JC ;
Freeman, AE ;
Kelley, DH ;
Stabel, JR ;
Kehrli, ME .
JOURNAL OF DAIRY SCIENCE, 1997, 80 (02) :400-405
[5]   Detection of bovine leukocyte antigen DRB3 alleles as candidate markers for clinical mastitis resistance in Holstein x Zebu [J].
Duangjinda, M. ;
Buayai, D. ;
Pattarajinda, V. ;
Phasuk, Y. ;
Katawatin, S. ;
Vongpralub, T. ;
Chaiyotvittayakul, A. .
JOURNAL OF ANIMAL SCIENCE, 2009, 87 (02) :469-476
[6]  
Esteban EN, 2009, ANIM SCI ISSUES PROF, P107
[7]   THE NUCLEOTIDE-SEQUENCE OF BOVINE MHC CLASS-II DQB AND DRB GENES [J].
GROENEN, MAM ;
VANDERPOEL, JJ ;
DIJKHOF, RJM ;
GIPHART, MJ .
IMMUNOGENETICS, 1990, 31 (01) :37-44
[8]   Association of BLV infection profiles with alleles of the BoLA-DRB3.2 gene [J].
Juliarena, M. A. ;
Poli, M. ;
Sala, L. ;
Ceriani, C. ;
Gutierrez, S. ;
Dolcini, G. ;
Rodriguez, E. M. ;
Marino, B. ;
Rodriguez-Dubra, C. ;
Esteban, E. N. .
ANIMAL GENETICS, 2008, 39 (04) :432-438
[9]   Genetic association between parameters of innate immunity and measures of mastitis in periparturient Holstein cattle [J].
Kelm, SC ;
Detilleux, JC ;
Freeman, AE ;
Kehrli, ME ;
Dietz, AB ;
Fox, LK ;
Butler, JE ;
Kasckovics, I ;
Kelley, DH .
JOURNAL OF DAIRY SCIENCE, 1997, 80 (08) :1767-1775
[10]   Study on the association of BoLA-DRB3.2 alleles with clinical mastitis in Norwegian Red cows [J].
Kulberg, S. ;
Heringstad, B. ;
Guttersrud, O. A. ;
Olsaker, I. .
JOURNAL OF ANIMAL BREEDING AND GENETICS, 2007, 124 (04) :201-207
123