Purification and properties of a chitinase from Penicillium sp LYG 0704

被引：61

作者：

Lee, Yoon Gyo ^{[1
]}

ChungB, Ki-Chul ^{[2
]}

Wi, Seung Gon ^{[3
]}

Lee, Jae Chang ^{[4
]}

Bae, Hyeun-Jong ^{[1
,3
]}

机构：

[1] Chonnam Natl Univ, BK21 Program, Dept Wood Sci & Engn, Kwangju 500757, South Korea

[2] Chonnam Natl Univ, Sch Biol Sci & Technol, Kwangju 500757, South Korea

[3] Chonnam Natl Univ, Bioenergy Res Inst, Kwangju 500757, South Korea

[4] Naju Coll, Dept Dent Hyg, Jollanam Do 520713, South Korea

来源：

PROTEIN EXPRESSION AND PURIFICATION | 2009年 / 65卷 / 02期

关键词：

Chitinase; Gene cloning; Penicillium sp; Purification; RACE-PCR; TRICHODERMA-HARZIANUM; ENDOCHITINASE; LYSOZYME; ENZYMES; CLUSTER; GENES;

D O I：

10.1016/j.pep.2008.12.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The chitinase producing Penicillium sp. LYG 0704 was procured from soil of the Chonnam National University crop field. The chitinase activity was detected after the first day which increased gradually and reached its maximum after 3 days of cultivation. The chitinase was purified from a culture medium by precipitation with isopropanol and column chromatography with Mono Q and Butyl-Sepha rose. The molecular mass of chitinase was estimated to be 47 kDa by SDS-PAGE. Optimal pH and temperature were 5.0 and 40 degrees C, respectively. The N-terminal amino acid sequence of the enzyme was determined to be (1)AGSYRSVAYFVDWAI(15). The fully cloned gene, 1287 bp in size, encoded a single peptide of 429 amino acids. BLAST search of the chitinase gene sequence showed similarity with chitinase of Aspergillus fumigatus Af293 chitinase gene (58%) and A. fumigatus class V chitinase ChiB1 gene (56%). (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：244 / 250

页数：7

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