Basis for structural diversity in homologous RNAs

被引:114
作者
Krasilnikov, AS
Xiao, YH
Pan, T
Mondragón, A
机构
[1] Northwestern Univ, Dept Biochem Mol Biol & Cell Biol, Evanston, IL 60208 USA
[2] Univ Chicago, Dept Biochem & Mol Biol, Chicago, IL 60637 USA
关键词
D O I
10.1126/science.1101489
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Large RNA molecules, such as ribozymes, fold with well-defined tertiary structures that are important for their activity. There are many instances of ribozymes with identical function but differences in their secondary structures, suggesting alternative tertiary folds. Here, we report a crystal structure of the 161-nucleotide specificity domain of an A-type ribonuclease P that differs in secondary and tertiary structure from the specificity domain of a B-type molecule. Despite the differences, the cores of the domains have similar three-dimensional structure. Remarkably, the similar geometry of the cores is stabilized by a different set of interactions involving distinct auxiliary elements.
引用
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页码:104 / 107
页数:4
相关论文
共 21 条
[1]  
Altman S., 1999, Cold Spring Harbor Monograph Archive, V37, P351
[2]   THE CCP4 SUITE - PROGRAMS FOR PROTEIN CRYSTALLOGRAPHY [J].
BAILEY, S .
ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1994, 50 :760-763
[3]   THE RIBONUCLEASE-P DATABASE [J].
BROWN, JW ;
HAAS, ES ;
GILBERT, DG ;
PACE, NR .
NUCLEIC ACIDS RESEARCH, 1994, 22 (17) :3660-3662
[4]  
Brunger AT, 1998, ACTA CRYSTALLOGR D, V54, P905, DOI 10.1107/s0907444998003254
[5]   Crystal structure of a group I ribozyme domain: Principles of RNA packing [J].
Cate, JH ;
Gooding, AR ;
Podell, E ;
Zhou, KH ;
Golden, BL ;
Kundrot, CE ;
Cech, TR ;
Doudna, JA .
SCIENCE, 1996, 273 (5282) :1678-1685
[6]  
Chen JL, 1997, RNA, V3, P557
[7]   Comparative photocross-linking analysis of the tertiary structures of Escherichia coli and Bacillus subtilis RNase P RNAs [J].
Chen, JL ;
Nolan, JM ;
Harris, ME ;
Pace, NR .
EMBO JOURNAL, 1998, 17 (05) :1515-1525
[8]   Maximum-likelihood heavy-atom parameter refinement for multiple isomorphous replacement and multiwavelength anomalous diffraction methods [J].
delaFortelle, E ;
Bricogne, G .
MACROMOLECULAR CRYSTALLOGRAPHY, PT A, 1997, 276 :472-494
[9]   Ribonuclease P: Unity and diversity in a tRNA processing ribozyme [J].
Frank, DN ;
Pace, NR .
ANNUAL REVIEW OF BIOCHEMISTRY, 1998, 67 :153-180
[10]   Structure and evolution of ribonuclease P RNA in gram-positive bacteria [J].
Haas, ES ;
Banta, AB ;
Harris, JK ;
Pace, NR ;
Brown, JW .
NUCLEIC ACIDS RESEARCH, 1996, 24 (23) :4775-4782