Production of transgenic chimeric rabbits and transmission of the transgene, through the germline

被引：9

作者：

Bodó, S

Gócza, E

Révay, T

Hiripi, L

Carstea, H

Kovács, A

Bodrogi, L

Bösze, Z

机构：

[1] Agr Biotechnol Ctr, Dept Biol Anim, H-2100 Godollo, Hungary

[2] Inst Small Anim Res, Godollo, Hungary

[3] Res Inst Anim Breeding & Nutr, Herceghalom, Hungary

来源：

MOLECULAR REPRODUCTION AND DEVELOPMENT | 2004年 / 68卷 / 04期

关键词：

reproductive technologies; developmental biology; germline chimeric rabbit; real-time PCR;

D O I：

10.1002/mrd.20109

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Here we report that improved reproductive technologies combined with an efficient microinjection method and in vitro cultivation medium enabled us to create germ line chimeric rabbits. To follow the fate of the chimeric embryo a blastomere marked with the human blood coagulation factor VIII (hFVIII) transgene was microinjected into a morula stage wild type embryo. The degree of chimerism in different tissues was estimated by real-time PCR and was found to be in the range of 0.1-42%. Among the four chimeric animals, one was identified as a chromosomal intersex and two were germline chimeras. (C) 2004 Wiley-Liss, Inc.

引用

页码：435 / 440

页数：6