A polarity dependent fluorescence "switch" in live cells

被引：27
作者：
Berns, MW
Krasieva, T
Sun, CH
Dvornikov, A
Rentzepis, PM
机构：
[1] Univ Calif Irvine, Beckman Laser Inst & Med Clin, Dept Biomed Engn, Irvine, CA 92612 USA
[2] Univ Calif Irvine, Dept Chem, Irvine, CA 92612 USA
来源：
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY | 2004年 / 75卷 / 1-2期
关键词：
photochromism; polarity; fluorescence; live cells; Bi-stable switch;
D O I：
10.1016/j.jphotobiol.2004.05.002
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
The spectroscopic properties, ultrafast kinetics and utilization of a photochromic molecule as a bi-stable fluorescing sensor of polarity in live cells are described. This molecule is a photochromic fulgimide, 2,3-dialkylidenesuccinimide, which emits fluorescence that can be switched optically on and off. The fluorescence intensity is a function of the polarity of the molecular environment, namely it fluoresces strongly when the molecule is in its polar isomeric structure form. We demonstrate that this molecule enters live cells without inducing damage, it binds primarily to internal membranous organelles, (mitochondria) and its fluorescence can be switched optically "on" and "off" repeatedly while inside the living cell. A possible use as a bi-stable, on/off sensor is discussed. (C) 2004 Published by Elsevier B.V.
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收藏
页码：51 / 56
页数：6
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