PKA regulatory subunit expression in tooth development

被引：4

作者：

de Sousa, Silvia Ferreira ^{[1
]}

Kawasaki, Katsushige ^{[2
]}

Kawasaki, Maiko ^{[2
]}

Volponi, Ana Angelova ^{[2
]}

Gomez, Ricardo Santiago ^{[1
]}

Gomes, Carolina Cavalieri ^{[3
]}

Sharpe, Paul T. ^{[2
]}

Ohazama, Atsushi ^{[2
,4
]}

机构：

[1] Univ Fed Minas Gerais, Sch Dent, Dept Oral Surg & Pathol, BR-31270901 Belo Horizonte, MG, Brazil

[2] Kings Coll London, Tower Wing Guys Hosp, Inst Dent, Dept Craniofacial Dev & Stem Cell Biol, London SE1 9RT, England

[3] Univ Fed Minas Gerais, Inst Biol Sci, Dept Pathol, BR-31270901 Belo Horizonte, MG, Brazil

[4] Niigata Univ, Grad Sch Med & Dent Sci, Dept Oral Biol Sci, Div Oral Anat, Niigata, Japan

来源：

GENE EXPRESSION PATTERNS | 2014年 / 15卷 / 01期

基金：

英国惠康基金;

关键词：

PRKAR1A; PRKAR2A; Tooth development; Human; Mouse; In situ hybridization; PROTEIN-KINASE-A; CYCLIC-AMP; GENE; PRKAR1A; PATHWAY; TUMORS; PHOSPHORYLATION; DIFFERENTIATION; ENDOCRINE; INCREASE;

D O I：

10.1016/j.gep.2014.04.002

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Protein kinase A (PK A) plays critical roles in many biological processes including cell proliferation, cell differentiation, cellular metabolism and gene regulation. Mutation in PKA regulatory subunit, PRKAR1A has previously been identified in odontogenic myxomas, but it is unclear whether PKA is involved in tooth development. The aim of the present study was to assess the expression of alpha isoforms of PICA regulatory subunit (Prkar1a and Prkar2a) in mouse and human odontogenesis by in situ hybridization. PRKAR1A and PRKAR2A mRNA transcription was further confirmed in a human deciduous germ by qRT-PCR. Mouse Prkar1a and human PRKAR2A exhibited a dynamic spatio-temporal expression in tooth development, whereas neither human PRKAR1A nor mouse Prkar2a showed their expression in odontogenesis. These isoforms thus showed different expression pattern between human and mouse tooth germs. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：46 / 51

页数：6

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