A method for super-resolved CARS microscopy with structured illumination in two dimensions

被引：27

作者：

Park, Joo Hyun ^{[1
,2
]}

Lee, Sang-Won ^{[2
]}

Lee, Eun Seong ^{[3
]}

Lee, Jae Yong ^{[1
,4
]}

机构：

[1] Univ Sci & Technol, Taejon 305350, South Korea

[2] Korea Res Inst Stand & Sci, Ctr NanoSafety, Taejon 304340, South Korea

[3] Korea Res Inst Stand & Sci, Ctr Nanometrol, Taejon 304340, South Korea

[4] Korea Res Inst Stand & Sci, Ctr Length, Taejon 304340, South Korea

来源：

OPTICS EXPRESS | 2014年 / 22卷 / 08期

基金：

新加坡国家研究基金会;

关键词：

STOKES-RAMAN SCATTERING; RESOLUTION LIMIT;

D O I：

10.1364/OE.22.009854

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We propose a structured illumination scheme for achieving widefield coherent anti-Stokes Raman scattering (CARS) microscopy with a resolution surpassing the diffraction limit in two dimensions (2D). By acquiring a set of coherent images of a sample with third-order nonlinear susceptibility illuminated by the phase-matched excitation field of square lattice patterns, a 2D super-resolution CARS image can be reconstructed. We derive a theoretical framework to describe the coherent image formation and reconstruction scheme for this structured illumination CARS imaging system and carry out numerical simulations to investigate its imaging performance. The results demonstrate that our method promises a particular benefit on CARS microscopy by adding the super-resolution capability to improve its 2D spatial resolution by a factor of approximately three. (C) 2014 Optical Society of America

引用

页码：9854 / 9870

页数：17

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