Three-dimensional culture of endometrial cells from domestic cats: A new in vitro platform for assessing plastic toxicity

被引：21
作者：
Dundon, Morgan [1 ,2 ,3 ]
Madden, Odile [2 ,4 ]
Comizzoli, Pierre [1 ]
机构：
[1] Natl Zool Pk, Ctr Species Survival, Conservat Biol Inst, Washington, DC 20008 USA
[2] Smithsonian Museum Conservat Inst, Suitland, MD USA
[3] Univ Calif Riverside, Mat Sci & Engn Program, Riverside, CA USA
[4] Getty Conservat Inst, Los Angeles, CA USA
来源：
PLOS ONE | 2019年 / 14卷 / 05期
关键词：
LONG-TERM; MODELS; IDENTIFICATION; LEACHABLES; ORGANOIDS; APOPTOSIS; CHEMICALS; MOUSE; ZINC;
D O I：
10.1371/journal.pone.0217365
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Plastic polymers can be combined with additives that modify physical properties and stability of the material. However, the biocompatibility of those additives is not well known. The objective of the study was to characterize the impact of zinc stearate-a common additivethrough the development of a novel three-dimensional (3-D) in vitro platform with endometrial cells from domestic cats. Epithelial and stromal cells from adult uteri were isolated and cultured in medium supplemented with 3% Matrigel for two weeks in plastic tissue culture dishes that had been identified as polystyrene with and without zinc stearate by Raman, FTIR, and X-ray fluorescence spectroscopies. Three-dimensional cell structures that were obtained were measured and categorized by shape. Cell viability, proliferation, differentiation, organization, and apoptosis then were assessed by immuno-staining. Results indicated that zinc stearate did not affect 3-D endometrial cell structure morphology, viability, or cellular composition. This first study of a new in vitro platform will be useful for studies testing the influence of other additives, drugs, or exogenous hormones.
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页数：15
相关论文
共 32 条
[1]  
[Anonymous], RTECS ZH5200000 ZINC
[2]   Endometrial stromal cells regulate epithelial cell growth in vitro:: a new co-culture model [J].
Arnold, JT ;
Kaufman, DG ;
Seppälä, M ;
Lessey, BA .
HUMAN REPRODUCTION, 2001, 16 (05) :836-845
[3]   Critical analysis of 3-D organoid in vitro cell culture models for high-throughput drug candidate toxicity assessments [J].
Astashkina, Anna ;
Grainger, David W. .
ADVANCED DRUG DELIVERY REVIEWS, 2014, 69 :1-18
[4]   Development of organoids from mouse and human endometrium showing endometrial epithelium physiology and long-term expandability [J].
Boretto, Matteo ;
Cox, Benoit ;
Noben, Manuel ;
Hendriks, Nikolai ;
Fassbender, Amelie ;
Roose, Heleen ;
Amant, Frederic ;
Timmerman, Dirk ;
Tomassetti, Carla ;
Vanhie, Arne ;
Meuleman, Christel ;
Ferrante, Marc ;
Vankelecom, Hugo .
DEVELOPMENT, 2017, 144 (10) :1775-1786
[5]   Gene expression analysis of human endometrial endothelial cells exposed to Bisphenol A [J].
Bredhult, Carolina ;
Sahlin, Lena ;
Olovsson, Matts .
REPRODUCTIVE TOXICOLOGY, 2009, 28 (01) :18-25
[6]   Assessing safety of extractables from materials and leachables in pharmaceuticals and biologics - Current challenges and approaches [J].
Broschard, Thomas H. ;
Glowienke, Susanne ;
Bruen, Uma S. ;
Nagao, Lee M. ;
Teasdale, Andrew ;
Stults, Cheryl L. M. ;
Li, Kim L. ;
Iciek, Laurie A. ;
Erexson, Greg ;
Martin, Elizabeth A. ;
Ball, Douglas J. .
REGULATORY TOXICOLOGY AND PHARMACOLOGY, 2016, 81 :201-211
[7]   Bisphenol a and the female reproductive tract: an overview of recent laboratory evidence and epidemiological studies [J].
Caserta, Donatella ;
Di Segni, Noemi ;
Mallozzi, Maddalena ;
Giovanale, Valentina ;
Mantovani, Alberto ;
Marci, Roberto ;
Moscarini, Massimo .
REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 2014, 12
[8]   Leachables from Saline-Containing IV Bags Can Alter Therapeutic Protein Properties [J].
Chang, Judy Y. ;
Xiao, Nina J. ;
Zhu, Min ;
Zhang, Jennifer ;
Hoff, Ed ;
Russell, Stephen J. ;
Katta, Viswanatham ;
Shire, Steven J. .
PHARMACEUTICAL RESEARCH, 2010, 27 (11) :2402-2413
[9]   Biomimicry 3D Gastrointestinal Spheroid Platform for the Assessment of Toxicity and Inflammatory Effects of Zinc Oxide Nanoparticles [J].
Chia, Sing Ling ;
Tay, Chor Yong ;
Setyawati, Magdiel I. ;
Leong, David T. .
SMALL, 2015, 11 (06) :702-712
[10]   Influence of warming and reanimation conditions on seminiferous tubule morphology, mitochondrial activity, and cell composition of vitrified testicular tissues in the domestic cat model [J].
Cruvinel Lima, David Baruc ;
Machado da Silva, Lucia Daniel ;
Comizzoli, Pierre .
PLOS ONE, 2018, 13 (11)
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