Preparation and biological evaluation of 99mTc-HYNIC-(Ser)3-D4 peptide for targeting and imaging of non-small-cell lung cancer

被引：15
作者：
Zahmatkesh, Mona Haddad [1 ]
Abedi, Seyed Mohammad [2 ,3 ]
Hosseinimehr, Seyed Jalal [1 ]
机构：
[1] Mazandaran Univ Med Sci, Dept Radiopharm, Fac Pharm, Sari, Iran
[2] Mazandaran Univ Med Sci, Dept Radiol, Fac Med, Sari, Iran
[3] Mazandaran Univ Med Sci, Cardiovasc Res Ctr, Sari, Iran
来源：
FUTURE ONCOLOGY | 2017年 / 13卷 / 10期
关键词：
Tc-99m; EGFR; non-small-cell lung cancer; peptide; tumor targeting; GROWTH-FACTOR RECEPTOR; RADIOCHEMICAL INVESTIGATIONS; VITRO/IN VIVO; TYROSINE KINASE; RENAL UPTAKE; LIGAND; ACID; EGFR; OVEREXPRESSION; DIAGNOSIS;
D O I：
10.2217/fon-2016-0426
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Aim: In this study, radiolabeled D4 peptide conjugate was studied as a radiotracer for imaging of non-small-cell lung cancer with overexpression of EGFR. Methods: HYNIC-(Ser) 3-D4 peptide was labeled with Tc-99m using tricine as a co-ligand. Cellular specific binding and internalization as well as in vivo tumor targeting were assessed. Results: The in vitro experiments showed good cellular specific binding. Tumor uptake values as % ID/g were 7.55 and 6.82% at 1 and 4 h after injection, respectively. The presaturation of EGFR in xenografted nude mice reduced 36% tumor uptake of radioactivity at 1 h after injection that confirmed in vivo specificity. Conclusion: Findings showed this radiolabeled peptide is a promising candidate for tumor targeting and molecular imaging of non-small-cell lung cancer.
引用
收藏
页码：893 / 905
页数：13
相关论文
共 43 条
[31]   Incorporation of a Triglutamyl Spacer Improves the Biodistribution of Synthetic Affibody Molecules Radiofluorinated at the N-Terminus via Oxime Formation with 18F-4-Fluorobenzaldehyde [J].
Rosik, Daniel ;
Thibblin, Alf ;
Antoni, Gunnar ;
Honarvar, Hadis ;
Strand, Joanna ;
Selvaraju, Ram Kumar ;
Altai, Mohamed ;
Orlova, Anna ;
Karlstrom, Arnelie Eriksson ;
Tolmachev, Vladimir .
BIOCONJUGATE CHEMISTRY, 2014, 25 (01) :82-92
[32]   Efficacy of 18F-fluorodeoxyglucose positron emission tomography/computed tomography as a predictor of response in locally advanced non-small-cell carcinoma of the lung [J].
Roy, Soumyajit ;
Pathy, Sushmita ;
Kumar, Rakesh ;
Mohanti, Bidhu K. ;
Raina, Vinod ;
Jaiswal, Anand ;
Taywade, Sameer ;
Garg, Kavita ;
Thulkar, Sanjay ;
Mohan, Anant ;
Mathur, Sandeep ;
Behera, Digamber .
NUCLEAR MEDICINE COMMUNICATIONS, 2016, 37 (02) :129-138
[33]   EPIDERMAL GROWTH FACTOR-RELATED PEPTIDES AND THEIR RECEPTORS IN HUMAN MALIGNANCIES [J].
SALOMON, DS ;
BRANDT, R ;
CIARDIELLO, F ;
NORMANNO, N .
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY, 1995, 19 (03) :183-232
[34]   Selective targeting of cancer cells using synthetic peptides [J].
Shadidi, M ;
Sioud, M .
DRUG RESISTANCE UPDATES, 2003, 6 (06) :363-371
[35]  
Smith CJ, 2003, CANCER RES, V63, P4082
[36]   Radiochemical investigations of 177Lu-DOTA-8-Aoc-BBN[7-14]NH2:: an in vitro/in vivo assessment of the targeting ability of this new radiopharmaceutical for PC-3 human prostate cancer cells [J].
Smith, CJ ;
Gali, H ;
Sieckman, GL ;
Hayes, DL ;
Owen, NK ;
Mazuru, DG ;
Volkert, WA ;
Hoffman, TJ .
NUCLEAR MEDICINE AND BIOLOGY, 2003, 30 (02) :101-109
[37]   Radiochemical investigations of 99mTc-N3S-X-BBN[7-14]NH2:: An in vitro/in vivo structure-activity relationship study where X = 0-, 3-, 5-, 8-, and 11-carbon tethering moieties [J].
Smith, CJ ;
Gali, H ;
Sieckman, GL ;
Higginbotham, C ;
Volkert, WA ;
Hoffman, TJ .
BIOCONJUGATE CHEMISTRY, 2003, 14 (01) :93-102
[38]   Novel peptide ligand directs liposomes toward EGF-R high-expressing cancer cells in vitro and in vivo [J].
Song, Shuxian ;
Liu, Dan ;
Peng, Jinliang ;
Deng, Hongwei ;
Guo, Yan ;
Xu, Lisa X. ;
Miller, Andrew D. ;
Xu, Yuhong .
FASEB JOURNAL, 2009, 23 (05) :1396-1404
[39]   In vivo evaluation of cysteine-based chelators for attachment of 99mTc to tumor-targeting affibody molecules [J].
Tran, Thuy ;
Engfeldt, Torun ;
Orlova, Anna ;
Widstrom, Charles ;
Bruskin, Alexander ;
Tolmachev, Vladimir ;
Karlstrom, Amelie Eriksson .
BIOCONJUGATE CHEMISTRY, 2007, 18 (02) :549-558
[40]   PROGNOSTIC-SIGNIFICANCE OF THE EXPRESSION OF C-FOS, C-JUN AND C-ERBB-1 ONCOGENE PRODUCTS IN HUMAN SQUAMOUS-CELL LUNG CARCINOMAS [J].
VOLM, M ;
DRINGS, P ;
WODRICH, W .
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, 1993, 119 (09) :507-510
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