Preparation and biological evaluation of 99mTc-HYNIC-(Ser)3-D4 peptide for targeting and imaging of non-small-cell lung cancer

被引：15
作者：
Zahmatkesh, Mona Haddad [1 ]
Abedi, Seyed Mohammad [2 ,3 ]
Hosseinimehr, Seyed Jalal [1 ]
机构：
[1] Mazandaran Univ Med Sci, Dept Radiopharm, Fac Pharm, Sari, Iran
[2] Mazandaran Univ Med Sci, Dept Radiol, Fac Med, Sari, Iran
[3] Mazandaran Univ Med Sci, Cardiovasc Res Ctr, Sari, Iran
来源：
FUTURE ONCOLOGY | 2017年 / 13卷 / 10期
关键词：
Tc-99m; EGFR; non-small-cell lung cancer; peptide; tumor targeting; GROWTH-FACTOR RECEPTOR; RADIOCHEMICAL INVESTIGATIONS; VITRO/IN VIVO; TYROSINE KINASE; RENAL UPTAKE; LIGAND; ACID; EGFR; OVEREXPRESSION; DIAGNOSIS;
D O I：
10.2217/fon-2016-0426
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Aim: In this study, radiolabeled D4 peptide conjugate was studied as a radiotracer for imaging of non-small-cell lung cancer with overexpression of EGFR. Methods: HYNIC-(Ser) 3-D4 peptide was labeled with Tc-99m using tricine as a co-ligand. Cellular specific binding and internalization as well as in vivo tumor targeting were assessed. Results: The in vitro experiments showed good cellular specific binding. Tumor uptake values as % ID/g were 7.55 and 6.82% at 1 and 4 h after injection, respectively. The presaturation of EGFR in xenografted nude mice reduced 36% tumor uptake of radioactivity at 1 h after injection that confirmed in vivo specificity. Conclusion: Findings showed this radiolabeled peptide is a promising candidate for tumor targeting and molecular imaging of non-small-cell lung cancer.
引用
收藏
页码：893 / 905
页数：13
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