DNA barcoding to map the microbial communities: current advances and future directions

被引:40
作者
Chakraborty, Chiranjib [1 ]
Doss, C. George Priya [2 ]
Patra, Bidhan C. [3 ]
Bandyopadhyay, Sanghamitra [4 ]
机构
[1] Galgotias Univ, Sch Comp & Informat Sci, Dept Bioinformat, Greater Noida, India
[2] VIT Univ, Sch Biosci & Technol, Med Biotechnol Div, Vellore 632014, Tamil Nadu, India
[3] Vidyasagar Univ, Dept Zool, Midnapore 721102, W Bengal, India
[4] Indian Stat Inst, Machine Intelligence Unit, Kolkata, India
关键词
Microbial community; DNA barcode; Next generation sequencing; Barcode marker gene; Biodiversity; 16S RIBOSOMAL-RNA; INTERNAL TRANSCRIBED SPACER; CYTOCHROME-C-OXIDASE; SPECIES DELIMITATION; CRYPTIC DIVERSITY; BACTERIAL IDENTIFICATION; BASIDIOMYCETOUS YEASTS; CLINICAL MICROBIOLOGY; MITOCHONDRIAL-DNA; GENE;
D O I
10.1007/s00253-014-5550-9
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
During the last two decades, the DNA barcode development towards microbial community has increased dramatically. DNA barcode development is related to error-free and quick species identification which aid in understanding the microbial biodiversity, as well as the diseases related to microbial species. Here, we seek to evaluate the so-called barcoding initiatives for the microbial communities and the emerging trends in this field. In this paper, we describe the development of DNA marker-based DNA barcoding system, comparison between routine species identification and DNA barcode, and microbial biodiversity and DNA barcode for microbial communities. Two major topics, such as the molecular diversity of viruses and barcode for viruses have been discussed at the same time. We demonstrate the current status and the maker of DNA barcode for bacteria, algae, fungi, and protozoa. Furthermore, we argue about the promises, limitations, and present and future challenges of microbial barcode development.
引用
收藏
页码:3425 / 3436
页数:12
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