Pluronic F-127 as a Cell Carrier for Bone Tissue Engineering

被引：29

作者：

Brunet-Maheu, Jean-Marc ^{[1
]}

Fernandes, Julio C. ^{[1
]}

de Lacerda, Clemente Av ^{[1
]}

Shi, Qin ^{[1
]}

Benderdour, Mohamed ^{[1
]}

Lavigne, Patrick ^{[1
,2
]}

机构：

[1] Ctr Hosp Sacre Coeur, Orthopaed Res Lab, Dept Orthopaed, Montreal, PQ, Canada

[2] Ctr Hosp Maisonneuve Rosemont, Dept Orthopaed, Montreal, PQ, Canada

来源：

JOURNAL OF BIOMATERIALS APPLICATIONS | 2009年 / 24卷 / 03期

关键词：

Pluronic F-127; Bone; Osteoblast; Tissue engineering; Platelet-Rich Plasma; PLATELET-RICH PLASMA; STEM-CELLS; IN-VITRO; REGENERATION; CARTILAGE; F127; PROSTAGLANDIN-E2; SUBSTITUTES; SCAFFOLDS; MEMBRANE;

D O I：

10.1177/0885328208096534

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The objective of this study is to report the effect of Pluronic F-127 on osteoblast viability and phenotype maintenance in vitro. MG-63 cells are suspended in Pluronic F-127, and MTT assay, alkaline phosphatase activity, prostaglandin E-2 production, collagen-I, and cyclo-oxygenase-2 expression are assessed up to 6 days. Pluronic F-127 leads to a significant decrease in osteoblast viability throughout the 6-day experiment, without altering osteoblast phenotype. The addition of platelet-rich plasma to the polymer/cell construct leads to increased cell survival. When supplemented with bioactive factors, Pluronic F-127 could potentially be used as a cell carrier in bone tissue engineering.

引用

页码：275 / 287

页数：13

共 27 条

[1]

Cao YL, 1998, J BIOMAT SCI-POLYM E, V9, P475

[2] Evaluation of pluronic polyols as carriers for grafting materials: Study in rat calvaria defects [J].

Fowler, EB ;

Cuenin, MF ;

Hokett, SD ;

Peacock, ME ;

McPherson, JC ;

Dirksen, TR ;

Sharawy, M ;

Billman, MA .

JOURNAL OF PERIODONTOLOGY, 2002, 73 (02) :191-197

[3]

Gazdag, 1995, J Am Acad Orthop Surg, V3, P1

[4]

Hollinger JO, 1996, CLIN ORTHOP RELAT R, P55

[5] Pluronic F127 as a cell encapsulation material: Utilization of membrane-stabilizing agents [J].

Khattak, SF ;

Bhatia, SR ;

Roberts, SC .

TISSUE ENGINEERING, 2005, 11 (5-6) :974-983

[6] Subchondral and trabecular bone metabolism regulation in canine experimental knee osteoarthritis [J].

Lavigne, P ;

Benderdour, M ;

Lajeunesse, D ;

Reboul, P ;

Shi, Q ;

Pelletier, JP ;

Martel-Pelletier, J ;

Fernandes, JC .

OSTEOARTHRITIS AND CARTILAGE, 2005, 13 (04) :310-317

[7]

Lin Sylvia S, 2006, Conf Proc IEEE Eng Med Biol Soc, V2006, P4358

[8] LOCAL INFUSION OF PROSTAGLANDIN-E1 STIMULATES MANDIBULAR BONE-FORMATION INVIVO [J].

MARKS, SC ;

MILLER, S .

JOURNAL OF ORAL PATHOLOGY & MEDICINE, 1988, 17 (9-10) :500-505

[9] Platelet-rich plasma - Growth factor enhancement for bone grafts [J].

Marx, RE ;

Carlson, ER ;

Eichstaedt, RM ;

Schimmele, SR ;

Strauss, JE ;

Georgeff, KR .

ORAL SURGERY ORAL MEDICINE ORAL PATHOLOGY ORAL RADIOLOGY AND ENDODONTICS, 1998, 85 (06) :638-646

[10]

Mylonas Dimitrios, 2007, J Prosthodont, V16, P421, DOI 10.1111/j.1532-849X.2007.00218.x

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