Identification of Novel Long Noncoding RNAs and Their Role in Abdominal Aortic Aneurysm

被引:8
作者
Maitiseyiti, Abulaihaiti [1 ]
Ci, Hongbo [2 ]
Fang, Qingbo [2 ]
Guan, Sheng [2 ]
Shawuti, Alimujiang [2 ]
Wang, Huguo [3 ]
Ge, Xiaohu [2 ]
机构
[1] Xinjiang Med Univ, Urumqi, Xinjiang Uygur, Peoples R China
[2] Peoples Hosp Xinjiang Uygur Autonomous Reg, Dept Vasc Surg, Urumqi, Xinjiang Uygur, Peoples R China
[3] Xinjiang Med Univ, Ctr Digest & Vasc Surg, Dept Vasc & Thyroid Surg, Affiliated Hosp 1, Urumqi, Xinjiang Uygur, Peoples R China
基金
中国国家自然科学基金;
关键词
EXPRESSION; PROLIFERATION; DISEASE; LNCRNAS; CERNA;
D O I
10.1155/2020/3502518
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Objective. Long noncoding RNAs (lncRNAs) have emerged as critical molecular regulators in various diseases. However, the potential regulatory role of lncRNAs in the pathogenesis of abdominal aortic aneurysm (AAA) remains elusive. The aim of this study was to identify crucial lncRNAs associated with human AAA by comparing the lncRNA and mRNA expression profiles of patients with AAA with those of control individuals. Materials and Methods. The expression profiles of lncRNAs and mRNAs were analyzed in five dilated aortic samples from AAA patients and three normal aortic samples from control individuals using microarray technology. Functional annotation of the screened lncRNAs based on the differentially expressed genes was performed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. Results. Microarray results revealed 2046 lncRNAs and 1363 mRNAs. Functional enrichment analysis showed that the mRNAs significantly associated with AAA were enriched in the NOD-like receptor (NLR) and nuclear factor kappa-B (NF-kappa B) signaling pathways and in cell adhesion molecules (CAMs), which are closely associated with pathophysiological changes in AAA. The lncRNAs identified using microarray analysis were further validated using quantitative real-time polymerase chain reaction (qRT-PCR) analysis with 12 versus 11 aortic samples. Finally, three key lncRNAs (ENST00000566954, ENST00000580897, and T181556) were confirmed using strict validation. A coding-noncoding coexpression (CNC) network and a competing endogenous RNA (ceRNA) network were constructed to determine the interaction among the lncRNAs, microRNAs, and mRNAs based on the confirmed lncRNAs. Conclusions. Our microarray profiling analysis and validation of significantly expressed lncRNAs between patients with AAA and control group individuals may provide new diagnostic biomarkers for AAA. The underlying regulatory mechanisms of the confirmed lncRNAs in AAA pathogenesis need to be determined using in vitro and in vivo experiments.
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页数:22
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