Automatic detection of hypoxia in renal tissue stained with HIF-1 alpha

被引:3
作者
Buzin, Aline Rodrigues [1 ]
Macedo, Nayana Damiani [1 ]
Binotti Abreu De Araujo, Isabela Bastos [2 ,3 ]
Nogueira, Breno Valentim [2 ]
de Andrade, Tadeu Uggere [1 ]
Endringer, Denise Coutinho [1 ]
Lenz, Dominik [1 ]
机构
[1] Univ Vila Velha, Pharmaceut Sci, Vila Velha, ES, Brazil
[2] Univ Fed Espirito Santo, Dept Morphol, Vitoria, ES, Brazil
[3] Tech Univ Dresden, Fac Med Carl Gustav Carus, Dresden, Germany
关键词
HIF l alpha; Hypoxia; Machine learning; Image analysis; SLIDE-BASED CYTOMETRY; THROUGHPUT IMAGE-ANALYSIS; LASER-SCANNING CYTOMETRY; INDUCIBLE FACTOR 1-ALPHA; BREAST-CANCER; QUANTIFICATION; MICROSCOPY; CARCINOMA; SOFTWARE; MARKERS;
D O I
10.1016/j.jim.2017.02.005
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Objective: The objective of this study was the identification of the stain HIF-alpha using the Image Cytometry, and to help to count the positive cells (with HIF-alpha) and the negative cells (without HIF-alpha) from the same sample. Method: 17 images of renal tissues from male rats of Winstar lineage; overall, there were 12.587 objects (cells) in the images for analysis. The acquired images were then analyzed through the free softwares CellProfiler (version 2.1.1) and CellProfiler Analyst (version 2.0). In the software CellProfiler Anlyst, there was a separation with the classes of the object, using a classifier, and the classes were: 1) class with HIF-alpha and 2) class without HIF-alpha. Results: With the data obtained through Score All, it was possible to calculate the percentage of cells that had HIFalpha; out of 12.587 objects of the sample, 6.773 (54%) had HIF-alpha and 5.814 (46%) did not have HIF-alpha. Data of sensibility 0.90, specificity 0.84 and standard deviation 0.10 and 0.12. Conclusion: The research shows that the free software CellProfiler, through the light microscope, was able to identify the stains, perform the machine's learning, and subsequently count and separate cells from distinct classes (with and without the stain of HIF-alpha). (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:47 / 50
页数:4
相关论文
共 33 条
[11]   Applications and challenges of digital pathology and whole slide imaging [J].
Higgins, C. .
BIOTECHNIC & HISTOCHEMISTRY, 2015, 90 (05) :341-347
[12]   Improved structure, function and compatibility for CellProfiler: modular high-throughput image analysis software [J].
Kamentsky, Lee ;
Jones, Thouis R. ;
Fraser, Adam ;
Bray, Mark-Anthony ;
Logan, David J. ;
Madden, Katherine L. ;
Ljosa, Vebjorn ;
Rueden, Curtis ;
Eliceiri, Kevin W. ;
Carpenter, Anne E. .
BIOINFORMATICS, 2011, 27 (08) :1179-1180
[13]   Prognostic assessment of hypoxia and metabolic markers in cervical cancer using automated digital image analysis of immunohistochemistry [J].
Kim, Bo Wook ;
Cho, Hanbyoul ;
Chung, Joon-Yong ;
Conway, Catherine ;
Ylaya, Kris ;
Kim, Jae-Hoon ;
Hewitt, Stephen M. .
JOURNAL OF TRANSLATIONAL MEDICINE, 2013, 11
[14]   Iterative restaining as a pivotal tool for n-color inununophenotyping by slide-based cytometry [J].
Laffers, W ;
Mittag, A ;
Lenz, D ;
Tárnok, A ;
Gerstner, AOH .
CYTOMETRY PART A, 2006, 69A (03) :127-130
[15]   CellProfilerTM:: free, versatile software for automated biological image analysis [J].
Lamprecht, Michael R. ;
Sabatini, David M. ;
Carpenter, Anne E. .
BIOTECHNIQUES, 2007, 42 (01) :71-75
[16]  
Lenz D, 2005, J BIOL REG HOMEOS AG, V19, P180
[17]   Regenerative and predictive medicine of cardiovascular disease:: The 9th Leipziger Workshop and the 2nd International Workshop on Slide Based Cytometry [J].
Lenz, D ;
Barten, MJ ;
Hiller, S ;
Tárnok, A ;
Sack, U .
CYTOMETRY PART A, 2005, 64A (02) :110-114
[18]   HIF-1alpha Expression Profile in Intratumoral and Peritumoral Inflammatory Cells as a Prognostic Marker for Squamous Cell Carcinoma of the Oral Cavity [J].
Mendes, Suzanny Oliveira ;
dos Santos, Marcelo ;
Peterle, Gabriela Tonini ;
Maia, Lucas de Lima ;
Stur, Elaine ;
Agostini, Lidiane Pignaton ;
de Carvalho, Marcos Brasilino ;
Tajara, Eloiza Helena ;
Louro, Iuri Drumond ;
Trivilin, Leonardo Oliveira ;
Alvares da Silva-Conforti, Adriana Madeira .
PLOS ONE, 2014, 9 (01)
[19]   Polychromatic (eight-color) slide-based cytometry for the phenotyping of leukocyte, NK, and NKT subsets [J].
Mittag, A ;
Lenz, D ;
Gerstner, AOH ;
Sack, U ;
Steinbrecher, M ;
Koksch, M ;
Raffael, A ;
Bocsi, J ;
Tárnok, A .
CYTOMETRY PART A, 2005, 65A (02) :103-115
[20]   Hyperchromatic cytometry principles for cytomics using slide based cytometry [J].
Mittag, Anja ;
Lenz, Dominik ;
Gerstner, Andreas O. H. ;
Tarnok, Attila .
CYTOMETRY PART A, 2006, 69A (07) :691-703