The administration of milk fermented by the probiotic Lactobacillus casei CRL 431 exerts an immunomodulatory effect against a breast tumour in a mouse model

被引:68
作者
Aragon, Felix [1 ]
Carino, Silvia [2 ]
Perdigon, Gabriela [1 ,3 ]
de Moreno de LeBlanca, Alejandra [1 ]
机构
[1] Consejo Nacl Invest Cient & Tecn, Ctr Referencia Lactobacilos CERELA, San Miguel De Tucuman, Tucuman, Argentina
[2] Univ Nacl Tucuman, Fac Odontol, Lab Anat Patol, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
[3] Univ Nacl Tucuman, Fac Bioquim Quim & Farm, Catedra Inmunol, RA-4000 San Miguel De Tucuman, Tucuman, Argentina
关键词
Angiogenesis; Breast cancer; Immune response; Mouse model; Probiotic; IMMUNE-SYSTEM; CANCER; CONSUMPTION; MUCOSAL; MACROPHAGES; ACTIVATION; HELVETICUS; PRODUCTS; STRAINS; IMPACT;
D O I
10.1016/j.imbio.2014.02.005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Antitumour activity is one of the health-promoting effects attributed to probiotics specially analysed from preclinical models, mostly murine. Here, the effect of milk fermented by the probiotic bacterium Lactobacillus casei CRL 431, on a murine breast cancer model was analysed. Mice were fed with milk fermented by Lactobacillus casei or unfermented milk before and after tumour injection. Rate of tumour development, cytokines in serum, IgA, CD4, CD8, F4/80 and cytokines positive cells in mammary glands were determined. Microvasculature in the tumour tissues was monitored. The effect of fermented milk administration after tumour injection was also evaluated. It was observed that probiotic administration delayed or blocked tumour development. This effect was associated to modulation of the immune response triggered by the tumour. The area occupied by blood vessels decreased in the tumours from mice given fermented milk which agrees with their small tumours, and fewer side effects. Finally, it was observed that probiotic administration after tumour detection was also beneficial to delay the tumour growth. In conclusion, we showed in this study the potential of milk fermented by the probiotic Lactobacillus casei CRL431 to stimulate the immune response against this breast tumour, avoiding or delaying its growth when it was preventively administrated and also when the administration started after tumour cells injection. (C) 2014 Elsevier GmbH. All rights reserved.
引用
收藏
页码:457 / 464
页数:8
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