Extraction, preliminary characterization and immunostimulatory activity in vitro of a polysaccharide isolated from Strongylocentrotus nudus eggs

被引：16

作者：

Ma, Yihua ^{[1
]}

Xing, Yingying ^{[1
]}

Mi, Huiwen ^{[1
]}

Guo, Zhenquan ^{[1
]}

Lu, Yuanyuan ^{[1
]}

Xi, Tao ^{[1
]}

机构：

[1] China Pharmaceut Univ, Dept Marine Pharm, Nanjing 210009, Jiangsu, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2014年 / 111卷

关键词：

Sea urchin; SEP-2; Response surface methodology; Characterization; Immunostimulatory activity; MACROPHAGE IMMUNOMODULATORY ACTIVITY; NITRIC-OXIDE; RAW264.7; CELLS; BETA-GLUCAN; INHIBITION; ACTIVATION;

D O I：

10.1016/j.carbpol.2014.04.010

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A new water-soluble polysaccharide (SEP-2), with a molecular weight of 6.78 x 10(5) Da, was isolated from Strongylocentrotus nudus eggs under the extraction conditions optimized by response surface methodology (RSM). Preliminary characterization of SEP-2 was performed by HPSEC and GC-MS, indicating that SEP-2 could be a D-glucan containing a (1 -> 4)-linked D-Glcp backbone with (1 -> 3)-linked D-Glcp side chains. In subsequent immunostimulatory studies, significantly enhanced ROS level, NO production and inflammatory cytokines secretion (IL-1 beta, IL-6, and TNF-alpha) were observed in SEP-2 treated murine macrophage cell line RAW264.7. These results suggest that SEP-2 might have the capability to enhance the microbicidal activity and killing function of macrophages through the polarization toward the pro-inflammatory M1 state. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：576 / 583

页数：8

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