Mixed immunosorbent for selective on-line trace enrichment and liquid chromatography of phenylurea herbicides in environmental waters

被引：21
作者：
MartinEsteban, A
Fernandez, P
Stevenson, D
Camara, C
机构：
[1] UNIV COMPLUTENSE MADRID,FAC CIENCIAS QUIM,DEPT QUIM ANALIT,E-28040 MADRID,SPAIN
[2] UNIV SURREY,ROBENS INST,GUILDFORD GU2 5XH,SURREY,ENGLAND
来源：
ANALYST | 1997年 / 122卷 / 10期
关键词：
phenylurea herbicides; immunosorbent; liquid chromatography; environmental waters;
D O I：
10.1039/a702828h
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
An immunosorbent containing antiisoproturon and antichlortoluron antibodies immobilised on aldehyde-activated silica was employed for the on-line preconcentration and liquid chromatography-diode array detection of several phenylureas. The efficiency of the coupling of the immunosorbent to the liquid chromatographic system and the characteristics of the immunosorbent were evaluated. The on-line system allowed the selective trace enrichment of chlortoluron, isoproturon, metobromuron, linuron and chlorbromuron at the 0.05-0.5 mu g l(-1) level in ground and river waters and provided detection limits in the range 0.01-0.03 mu g l(-1) by percolating only 10 ml of water sample. The proposed method was validated by analysing freeze-dried tap water samples with a high content in pesticides of different chemical functionalities. The results obtained mere compared with the mean value obtained in an interlaboratory exercise.
引用
收藏
页码：1113 / 1117
页数：5
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