Real-time and wide-field mapping of cell-substrate adhesion gap and its evolution via surface plasmon resonance holographic microscopy

被引：20

作者：

Dai, Siqing ^{[1
,2
]}

Yu, Tongyao ^{[3
]}

Zhang, Jiwei ^{[1
,2
]}

Lu, Hua ^{[1
,2
]}

Dou, Jiazhen ^{[1
,2
]}

Zhang, Mengmeng ^{[1
,2
]}

Dong, Chen ^{[1
,2
]}

Di, Jianglei ^{[1
,2
]}

Zhao, Jianlin ^{[1
,2
]}

机构：

[1] Northwestern Polytech Univ, MOE Key Lab Mat Phys & Chem Extraordinary Condit, Sch Phys Sci & Technol, Xian 710129, Peoples R China

[2] Northwestern Polytech Univ, Shaanxi Key Lab Opt Informat Technol, Sch Phys Sci & Technol, Xian 710129, Peoples R China

[3] Northwestern Polytech Univ, Inst Special Environm Biophys, Sch Life Sci, Key Lab Space Biosci & Biotechnol, Xian 710072, Peoples R China

来源：

BIOSENSORS & BIOELECTRONICS | 2021年 / 174卷

基金：

中国国家自然科学基金;

关键词：

Living cell sensing; Surface plasmon resonance; Digital holographic microscopy; Cell-substrate adhesion gap; Bottom membrane dynamics; REFLECTION FLUORESCENCE MICROSCOPY; MATERIAL INTERFACE; CONTACTS; PHASE; FIBROBLASTS; MIGRATION; CONTRAST; BINDING;

D O I：

10.1016/j.bios.2020.112826

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

As one of the most common biological phenomena, cell adhesion plays a vital role in the cellular activities such as the growth and apoptosis, attracting tremendous research interests over the past decades. Taking the cell evolution under drug injection as an example, the dynamics of cell-substrate adhesion gap can provide valuable information in the fundamental research of cell contacts. A robust technique of monitoring the cell adhesion gap and its evolution in real time is highly desired. Herein, we develop a surface plasmon resonance holographic microscopy to achieve the novel functionality of real-time and wide-field mapping of the cell-substrate adhesion gap and its evolution in situ. The cell adhesion gap images of mouse osteoblast cells and human breast cancer cells have been effectively extracted in a dynamic and label-free manner. The proposed technique opens up a new avenue of revealing the cell-substrate interaction mechanism and renders the wide applications in the biosensing area.

引用

页数：8

共 51 条

[1] CELL-SUBSTRATE CONTACTS ILLUMINATED BY TOTAL INTERNAL-REFLECTION FLUORESCENCE [J].