On the Function of Pentameric Phospholamban: Ion Channel or Storage Form?

被引：34

作者：

Becucci, Lucia ^{[3
]}

Cembran, Alessandro ^{[1
]}

Karim, Christine B. ^{[2
]}

Thomas, David D. ^{[2
]}

Guidelli, Rolando ^{[3
]}

Gao, Jiali ^{[1
]}

Veglia, Gianluigi ^{[1
,2
]}

机构：

[1] Univ Minnesota, Dept Chem, Minneapolis, MN 55455 USA

[2] Univ Minnesota, Dept Biochem Mol Biol & Biophys, Minneapolis, MN 55455 USA

[3] Univ Florence, Dept Chem, Florence, Italy

来源：

BIOPHYSICAL JOURNAL | 2009年 / 96卷 / 10期

基金：

美国国家卫生研究院;

关键词：

MOLECULAR-DYNAMICS; REGULATORY COMPLEX; MEMBRANE BILAYERS; ENERGY-TRANSFER; CALCIUM-PUMP; TRANSMEMBRANE; OLIGOMERIZATION; PERMEATION; REVEALS;

D O I：

10.1016/j.bpj.2009.03.013

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

Phospholamban (PLN) is an integral membrane protein that inhibits the sarcoplasmic reticulum Ca2+-ATPase, thereby regulating muscle contractility. We report a combined electrochemical and theoretical study demonstrating that the pentameric PLN does not possess channel activity for conducting chloride or calcium ions across the lipid membrane. This suggests that the pentameric configuration of PLN primarily serves as a storage form for the regulatory function of muscle relaxation by the PLN monomer.

引用

页码：L60 / L62

页数：3

共 25 条

[1] Molecular dynamics - potential of mean force calculations as a tool for understanding ion permeation and selectivity in narrow channels [J].