Extraction of cage-like sporopollenin exine capsules from dandelion pollen grains

被引:37
|
作者
Fan, Tengfei [1 ,2 ]
Park, Jae Hyeon [1 ,2 ]
Quynh Anh Pham [1 ,2 ]
Tani, Ee-Lin [1 ,2 ]
Mundargi, Raghavendra. C. [1 ,2 ]
Potroz, Michael G. [1 ,2 ]
Jung, Haram [1 ,2 ]
Cho, Nam-Joon [1 ,2 ,3 ]
机构
[1] Nanyang Technol Univ, Sch Mat Sci & Engn, 50 Nanyang Dr, Singapore 637553, Singapore
[2] Nanyang Technol Univ, Ctr Biomimet Sensor Sci, 50 Nanyang Dr, Singapore 637553, Singapore
[3] Nanyang Technol Univ, Sch Chem & Biomed Engn, 62 Nanyang Dr, Singapore 637459, Singapore
来源
SCIENTIFIC REPORTS | 2018年 / 8卷
基金
新加坡国家研究基金会;
关键词
DELIVERY; ENCAPSULATION; IBUPROFEN; RELEASE; SPORE;
D O I
10.1038/s41598-018-24336-9
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Pollen-based microcapsules such as hollow sporopollenin exine capsules (SECs) have emerged as excellent drug delivery and microencapsulation vehicles. To date, SECs have been extracted primarily from a wide range of natural pollen species possessing largely spherical geometries and uniform surface features. Nonetheless, exploring pollen species with more diverse architectural features could lead to new application possibilities. One promising class of candidates is dandelion pollen grains, which possess architecturally intricate, cage-like microstructures composed of robust sporopollenin biopolymers. Here, we report the successful extraction and macromolecular loading of dandelion SECs. Preservation of SEC morphology and successful removal of proteinaceous materials was evaluated using scanning electron microscopy (SEM), matrix-assisted laser desorption/ionization-time of flight (MALDI-TOF) mass spectrometry, elemental CHN analysis, dynamic image particle analysis (DIPA) and confocal laser scanning microscopy (CLSM). Among the tested processing schemes, acidolysis using 85% (v/v) phosphoric acid refluxed at 70 degrees C for 5 hours yielded an optimal balance of intact particle yield, protein removal, and preservation of cage-like microstructure. For proof-of-concept loading, bovine serum albumin (BSA) was encapsulated within the dandelion SECs with high efficiency (32.23 +/- 0.33%). Overall, our findings highlight how hollow microcapsules with diverse architectural features can be readily prepared and utilized from plant-based materials.
引用
收藏
页数:11
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