C5a Receptor Mediates Neutrophil Activation and ANCA-Induced Glomerulonephritis

被引:329
作者
Schreiber, Adrian [1 ]
Xiao, Hong [2 ]
Jennette, J. Charles [2 ]
Schneider, Wolfgang [1 ]
Luft, Friedrich C. [1 ]
Kettritz, Ralph [1 ]
机构
[1] HELIOS Klin, Max Delbruck Ctr Mol Med, Franz Volhard Clin, Fac Med Charite,Dept Nephrol & Hypertens, Berlin, Germany
[2] Univ N Carolina, Dept Pathol & Lab Med, Chapel Hill, NC USA
来源
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY | 2009年 / 20卷 / 02期
关键词
ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES; HUMAN POLYMORPHONUCLEAR LEUKOCYTES; ANTIMYELOPEROXIDASE ANTIBODIES; COMPLEMENT ACTIVATION; ENDOTHELIAL-CELLS; HYDROGEN-PEROXIDE; VASCULITIS; PROTEIN; MYELOPEROXIDASE; C5L2;
D O I
10.1681/ASN.2008050497
中图分类号
R5 [内科学]; R69 [泌尿科学(泌尿生殖系疾病)];
学科分类号
1002 ; 100201 ;
摘要
Anti-neutrophil cytoplasmic autoantibody (ANCA)-induced necrotizing crescentic glomerulonephritis (NCGN) requires complement participation in its pathogenesis. We tested the hypothesis that the anaphylatoxin C5a is pivotal to disease induction via the neutrophil C5a receptor (C5aR). Supernatants from ANICA-activated neutrophils activated the complement cascade in normal serum, producing C5a. This conditioned serum primed neutrophils for ANCA-induced respiratory burst; neutrophil C5aR blockade abrogated this priming, but C3aR blockade did not. Furthermore, recombinant C5a but not C3a dosage-dependently primed neutrophils for ANCA-induced respiratory burst. To test the role of C5aR in a model of NCGN, we immunized myeloperoxidase-deficient mice with myeloperoxidase, irradiated them, and transplanted bone marrow from wild-type mice or C5aR-deficient mice into them. All mice that received wild-type marrow (six of six) but only one of eight mice that received C5aR-deficient marrow developed NCGN (P < 0.05). Albuminuria and neutrophil influx into glomeruli were also significantly attenuated in the mice that received C5aR-deficient marrow (P < 0.05). In summary, C5a and the neutrophil C5aR may compose an amplification loop for ANCA-mediated neutrophil activation. The C5aR may provide a new therapeutic target for ANCA-induced necrotizing crescentic glomerulonephritis.
引用
收藏
页码:289 / 298
页数:10
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