Analysis of Protein Oxidative Damage and Pregnancy Associated Plasma Protein-A (PAPP-A) Expression in Preeclamptic Placenta

Preeclampsia is the biggest cause of maternal and fetal mortality. Ischemia Uteroplacental that cause oxidative stress in preeclampsia lead to dysfunction of placental cells. PAPP-A is an enzyme to cleaved the bonding between the IGF binding protein and IGF, free IGF causes trophoblast cells in order to improve placental tissue. The PAPP-A expression and its relationship to oxidative stress is still in question. This study was conducted using 34 samples each of preeclampsia and normal placental tissue. mRNA relative expression of PAPP- A was measured by qRT-PCR, protein PAPP-A was measured by ELISA. Carbonyl measured by dinitrofenil hydrazine method. The mother age was 30 +/- 76 +/- 6.0 years; gestational age 39 (26-42) weeks. The mRNA relative expression of PAPP- A was lower in the pre-eclampsia. PAPP- A protein levels preeclampsia groups 0.101 (0.048 to 0.425) ng/mL; normal group 0.066 (0.044 to 0.178) ng/mL. Elevated levels of PAPP- A protein is not statistically significant. Placenta Carbonyl has no significant difference between normal and preeclampsia. There were no significant differences between PAPP- A protein and carbonyl in preeclampsia and normal placental, but there is a significant negative correlation between the levels of PAPP- A and carbonyl in preeclampsia placenta (p = 0.003, r =- 0.354).