Regeneration and molecular analysis of date palm (Phoenix dactylifera L.) plantlets using RAPD markers

被引：0

作者：

Ahmed, Othmani ^{[1
]}

Chokri, Bayoudh ^{[2
]}

Noureddine, Drira

Mohamed, Marrakchi ^{[1
]}

Mokhtar, Trifi ^{[1
]}

机构：

[1] Fac Sci Tunis, Lab Genet Mol Immunol & Biotechnol, Tunis 1060, Tunisia

[2] Ctr Reg Rech Agr Oasienne, Lab Culture Vitro, Degache 2260, Tunisia

来源：

AFRICAN JOURNAL OF BIOTECHNOLOGY | 2009年 / 8卷 / 05期

关键词：

Date palm; 2,4-dichlorophenoxyacetic acid; random amplified polymorphic DNA; somaclonal variation; somatic embryogenesis; SOMATIC EMBRYOGENESIS; SOMACLONAL VARIATION; CULTURES;

D O I：

暂无

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Clonal plants of date palm (Poenix dactylifera L.) were regenerated from juvenile leaves on regimes consisting of the use of 2,4-D. Success depended on the concentrations of 2,4-D tested. The cultures produced adventitious shoot buds directly at the basal part of leaves as well as excessive calli. Somaclonal variation in plantlets which can be induced by 2,4-D during recurrent somatic embryogenesis were tested by random amplified polymorphic DNA ( RAPD) profiles. Nine arbitrary 10-mer primers were used to amplify DNA from 180 plantlets. RAPD patterns of the plantlets were identical with the original plant mother, indicating that 2,4-D did not induce somaclonal variation that can be detected by the RAPD technique.

引用

页码：813 / 820

页数：8

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