A bimodal molecular imaging probe based on chitosan encapsulated magneto-fluorescent nanocomposite offers biocompatibility, visualization of specific cancer cells in vitro and lung tissues in vivo

被引：18

作者：

Walia, Shanka ^{[1
,3
]}

Sharma, Supriya ^{[2
,3
]}

Kulurkar, Pankaj Markand ^{[2
]}

Patial, Vikram ^{[2
,3
]}

Acharya, Amitabha ^{[1
,3
]}

机构：

[1] CSIR Inst Himalayan Bioresource Technol, Div Biotechnol, Palampur 176061, HP, India

[2] CSIR Inst Himalayan Bioresource Technol, Pharmacol & Toxicol Lab, Food Nutraceut & Qual Control Div, Palampur 176061, HP, India

[3] Acad Sci & Innovat Res AcSIR, New Delhi, India

来源：

INTERNATIONAL JOURNAL OF PHARMACEUTICS | 2016年 / 498卷 / 1-2期

关键词：

Chitosan nanoparticles; Fluorescence imaging; Perl's Prussian blue staining; Histopathology; Hematology; IRON-OXIDE NANOPARTICLES; MRI CONTRAST AGENTS; DRUG-DELIVERY; QUANTUM DOTS; CYTOTOXICITY; TOXICITY; DESIGN;

D O I：

10.1016/j.ijpharm.2015.12.011

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Multifunctional hybrid nanocomposite material, consists of chitosan encapsulated iron oxide (as MRI contrasting agent), CdS (as fluorescent probe) nanoparticles and podophyllotoxin (as anticancer drug) was synthesized and characterized. The TEM studies suggested the size of the NPs to be in the range of 80-100 nm. These nanocomposites were treated with different cancer cell lines viz., KB, C6 and A549 cells. Fluorescence imaging and Perl's Prussian blue staining confirmed the presence of these nanocomposites inside both KB and C6 cells but not in A549 cells. Cytotoxicity experiments revealed that these biopolymer coated nanocomposites showed minimal toxicity towards cancerous cells. Further the intraperitoneal administration of one of the nanoformulations to Wistar rats suggested deposition of these nanocomposites in the lungs. The hematological, biochemical and histopathological analysis confirmed that these nanocomposites are safe to use as a novel dual mode imaging material. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：110 / 118

页数：9

共 59 条

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