Effect of angiotensin-II on renal Na+/H+ exchanger-NHE3 and NHE2

被引：42

作者：

Dixit, MP ^{[1
]}

Xu, LP ^{[1
]}

Xu, H ^{[1
]}

Bai, LQ ^{[1
]}

Collins, JF ^{[1
]}

Ghishan, FK ^{[1
]}

机构：

[1] Univ Arizona, Hlth Sci Ctr, Steel Mem Childrens Res Ctr, Dept Pediat & Physiol, Tucson, AZ 85724 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES | 2004年 / 1664卷 / 01期

关键词：

Na+/H+ exchange; proximal tubule; kidney; sodium uptake;

D O I：

10.1016/j.bbamem.2004.03.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The purpose of the present study was to determine the effect of angiotensin II (A-II) on membrane expression of Na+/H+ exchange isoforms NHE3 and NHE2 in the rat renal cortex. A-II (500 ng/kg per min) was chronically infused into the Sprague-Dawley rats by miniosmotic pump for 7 days. Arterial pressure and circulating plasma A-II level were significantly increased in A-II rats as compared to control rats. pH-dependent uptake of Na-22(+) study in the presence of 50 muM HOE-694 revealed that Na+ uptake mediated by NHE3 was increased similar to88% in the brush border membrane from renal cortex of A-II-treated rats. Western blotting showed that A-II increased NHE3 immunoreactive protein levels in the brush border membrane of the proximal tubules by 31%. Northern blotting revealed that A-II increased NHE3 mRNA abundance in the renal cortex by 42%. A-II treatment did not alter brush border NHE2 protein abundance in the renal proximal tubules. In conclusion, chronic A-II treatment increases NHE3-mediated Na+ uptake by stimulating NHE3 mRNA and protein content. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：38 / 44

页数：7

共 38 条

[1] Regulation of NHE3 activity by G protein subunits in renal brush-border membranes [J].