Genetic diversity of the giant tiger shrimp (Penaeus monodon) in Thailand revealed by PCR-SSCP of polymorphic EST-derived markers

被引：9
|
作者：
Klinbunga, Sirawut [1 ]
Preechaphol, Rachanimuk
Thumrungtanakit, Supaporn
Leelatanawit, Rungnapa
Aoki, Takashi
Jarayabhand, Padermsak
Menasveta, Piamsak
机构：
[1] Chulalongkorn Univ, Dept Marine Sci, Ctr Excellence Marine Biotechnol, Fac Sci, Bangkok 10330, Thailand
[2] Natl Sci & Technol Dev Agcy, Natl Ctr Genet Engn & Biotechnol, Pathum Thani 12120, Thailand
[3] Chulalongkorn Univ, Fac Sci, Program Biotechnol, Bangkok 10330, Thailand
[4] Tokyo Univ Marine Sci & Technol, Grad Sch Marine Sci & Technol, Lab Genome Sci, Tokyo 1008477, Japan
[5] Chulalongkorn Univ, Aquat Resources Res Inst, Bangkok 10330, Thailand
[6] Chulalongkorn Univ, Fac Sci, Dept Marine Sci, Bangkok 10330, Thailand
来源：
BIOCHEMICAL GENETICS | 2006年 / 44卷 / 5-6期
关键词：
genetic diversity; SSCP; EST; Penaeus monodon; shrimp;
D O I：
10.1007/s10528-006-9027-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A total of 90 ESTs from normal and 157 from subtractive ovarian cDNA libraries of the giant tiger shrimp (Penaeus monodon) were sequenced. SSCP analysis of disulfide isomerase (DSI), zinc finger protein (ZFP), PMO920, and PMT1700 was carried out for population genetic studies of P. monodon it? Thai waters. The number of codominant alleles per locus for overall samples was 6 for PM0920, 5 for PMT 1700, and 12 for ZFP, and there were 19 dominant alleles for DSI. The observed heterozygosity of each geographic sample was 0.3043-0.5128 for PM0920, 0.3462-0.4643 for PMT 1700, and 0.5000-0.8108 for ZFP. Linkage disequilibrilan analysis indicated that genotypes of these loci segregate randomly (P > 0.05). Low genetic distance was found between pairs of geographic samples (0.0077-0.0178). The neighbor-joining tree constructed from the average genetic distance of overall loci
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页码：222 / 236
页数：15
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