Antimicrobial peptides from Capsicum chinense fruits: agronomic alternatives against phytopathogenic fungi

被引：15

作者：

dos Santos, Layrana de Azevedo ^{[1
]}

Taveira, Gabriel Bonan ^{[1
]}

da Silva, Marciele Souza ^{[1
]}

Gebara, Rodrigo da Silva ^{[1
]}

Pereira, Lidia da Silva ^{[1
]}

Perales, Jonas ^{[2
]}

Teixeira-Ferreira, Andre ^{[2
]}

Mello, Erica de Oliveira ^{[1
]}

Carvalho, Andre de Oliveira ^{[1
]}

Rodrigues, Rosana ^{[3
]}

Gomes, Valdirene Moreira ^{[1
]}

机构：

[1] Univ Estadual Norte Fluminense, Ctr Biociencias & Biotecnol, Lab Fisiol & Bioquim Microrganismos, Campos Dos Goytacazes, RJ, Brazil

[2] Fundacao Osvaldo Cruz FIOCRUZ, Lab Toxinol, Rio De Janeiro, RJ, Brazil

[3] Univ Estadual Norte Fluminense, Ctr Ciencias & Tecnol Agr, Lab Melhoramento & Genet Vegetal, Campos Dos Goytacazes, RJ, Brazil

来源：

BIOSCIENCE REPORTS | 2020年 / 40卷

关键词：

PLANT DEFENSINS; MEMBRANE PERMEABILIZATION; PEPPERS CAPSICUM; OXIDATIVE STRESS; MECHANISM; PROTEINS; SEEDS; RESISTANCE; PATHOGENS; LECTIN;

D O I：

10.1042/BSR20200950

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In recent years, the antimicrobial activity of peptides isolated from a wide variety of organs from plant species has been reported. However, a few studies have investigated the potential of antimicrobial peptides (AMPs) found in fruits, especially Capsicum chinense (pepper). The present study aimed to purify and characterize peptides from Capsicum chinense fruits and evaluate their inhibitory activities against different phytopathogenic fungi and also analyze the possible mechanisms of action involved in microbial inhibition. After fruit protein extraction and high-performance liquid chromatography (HPLC), different fractions were obtained, named F1 to F10. Peptides in the F4 and F5 fractions were sequenced and revealed similarity with the plant antimicrobial peptides like non-specific lipid transfer proteins and defensin-like peptide. The F4 and F5 fractions presented strong antimicrobial activity against the fungus Fusarium solani and Fusarium oxysporum, causing toxic effects on these fungi, leading to membrane permeabilization, endogenous reactive oxygen species increase, activation of metacaspase and loss of mitochondrial function.

引用

页数：15

共 49 条

[1]

Aerts A.M., 2011, FRONT MICROBIOL, V47, P188

[2] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].