Preparations and doxorubicin controlled release of amino-acid based redox/pH dual-responsive nanomicelles

Terpolymers of poly (Lysine-co-N, N-Bis (acryloyl) cystamine-co-beta-Phenethylamine) (PLBP) were synthesized in one-pot by Michael addition terpolymerization. The terpolymers self-assembled into nano-sized spherical micelles (84-123 nm) with narrow distributions. The surface charge of the nanomicelles (NMs) was depended on solution's pH and showed negative values under physiological conditions (pH 7.4), which was beneficial for long circulation without non-specific protein adsorption. Doxorubicin (DOX) was effectively loaded into the NMs for controlled release. The in vitro release profiles exhibited obvious pH and reduction sensitivities in response to the environment mimicking tumor cells. The mir assays demonstrated that blank NMs were biocompatible, and drug-laden NMs showed a significant cytotoxicity on Hela cells. The NMs could be potentially applied as smart drug delivery systems in cancer therapy. (C) 2017 Elsevier B.V. All rights reserved.