Simultaneous Multicolor Luciferase Reporter Assays for Monitoring of Multiple Genes Expressions

被引：21

作者：

Ohmiya, Yoshihiro ^{[1
]}

机构：

[1] Natl Inst Adv Ind Sci & Technol, Biomed Res Inst, DAILAB, Tsukuba, Ibaraki, Japan

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2015年 / 18卷 / 10期

关键词：

Beetle luciferase; bioluminescence; multicolor luciferase assay; imaging luciferase assay; reporter assay; BIOLUMINESCENT REPORTER; FIREFLY OXYLUCIFERIN; SECRETED LUCIFERASE; QUANTUM YIELD; IN-VITRO; GREEN; CLONING; RED; MECHANISM; OXIDATION;

D O I：

10.2174/1386207318666150917095903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Multicolor luciferase reporter assays are powerful tools for simultaneously monitoring the expression of several genes. Dual-color luciferase reporter assays were established in 2004 and have been used in broad range of application from basic researches to applications in clinical research, e.g., drug screening and alternative toxicological tests. The advantage of this method is that it can be used to simultaneously monitor sequential and complex changes in the expression of multiple genes from the single-cell level to the tissue level. In this review, I focus on the simultaneous analysis of multiple genes using multicolor beetle luciferases.

引用

页码：937 / 945

页数：9

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