Comparison of methods to assess the enzyme accessibility and hydrolysis of pretreated lignocellulosic substrates

被引：66

作者：

Chandra, Richard P. ^{[1
]}

Ewanick, Shannon M. ^{[1
]}

Chung, Pablo A. ^{[1
]}

Au-Yeung, Kathy ^{[1
]}

Del Rio, Luis ^{[1
]}

Mabee, Warren ^{[1
]}

Saddler, Jack N. ^{[1
]}

机构：

[1] Univ British Columbia, Fac Forestry, Dept Wood Sci, Vancouver, BC BC V6T, Canada

来源：

BIOTECHNOLOGY LETTERS | 2009年 / 31卷 / 08期

基金：

加拿大自然科学与工程研究理事会;

关键词：

Bioconversion; Cellulase; Ethanol; Simons' stain; STAINING TECHNIQUE; LODGEPOLE PINE; CELLULASE; ETHANOL; BIOMASS;

D O I：

10.1007/s10529-009-9993-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Fiber size analysis, water retention value, and Simons' stain measurements were assessed for their potential to predict the susceptibility of a given substrate to enzymatic hydrolysis. Slight modifications were made to the fiber size analysis and water retention protocols to adapt these measurements to evaluate substrates for cellulolytic hydrolysis rather than pulps for papermaking. Lodgepole pine was pretreated by the steam and ethanol-organosolv processes under varying conditions. The Simons' stain procedure proved to be an effective method for indicating the potential ease of enzymatic hydrolysis of substrates pretreated by either process or when the pretreatment conditions were altered.

引用

页码：1217 / 1222

页数：6

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