A Strategy for Simultaneous Isolation of Less Polar Ginsenosides, Including a Pair of New 20-Methoxyl Isomers, from Flower Buds of Panax ginseng

被引：15

作者：

Li, Sha-Sha ^{[1
]}

Li, Ke-Ke ^{[1
]}

Xu, Fei ^{[1
]}

Tao, Li ^{[1
]}

Yang, Li ^{[2
]}

Chen, Shu-Xiao ^{[2
]}

Gong, Xiao-Jie ^{[1
]}

机构：

[1] Dalian Univ, Coll Med, Dalian 116622, Peoples R China

[2] Dalian Univ, Coll Environm & Chem Engn, Dalian 116622, Peoples R China

来源：

MOLECULES | 2017年 / 22卷 / 03期

关键词：

20(S)-methoxyl-ginsenoside Rg(3); 20(R)-methoxyl-ginsenoside Rg(3); isomer; quantification; flower buds of Panax ginseng; DAMMARANE-TYPE SAPONINS; EFFICIENT DISCOVERY; NATURAL COMPOUNDS; LEAVES; STEMS; CHROMATOGRAPHY; CONSTITUENTS; PHARMACOLOGY; LEAF; L;

D O I：

10.3390/molecules22030442

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study was designed to simultaneously isolate the less polar ginsenosides from the flower buds of Panax ginseng (FBPG). Five ginsenosides, including a pair of new 20-methoxyl isomers, were extracted from FBPG and purified through a five-step integrated strategy, by combining ultrasonic extraction, Diaion Hp-20 macroporous resin column enrichment, solid phase extraction (SPE), reversed-phase high-performance liquid chromatography (RP-HPLC) analysis and preparation, and nuclear magnetic resonance (NMR) analysis. The quantification of the five ginsenosides was also discussed by a developed method with validations within acceptable limits. Ginsenoside Rg(5) showed content of about 1% in FBPG. The results indicated that FBPG might have many different ginsenosides with diverse chemical structures, and the less polar ginsenosides were also important to the quality control and standardization of FBPG.

引用

页数：14