Transcription and nuclear transport of CAG/CTG trinucleotide repeats in yeast

被引：16

作者：

Fabre, E

Dujon, B

Richard, GF

机构：

[1] Univ Paris 06, Inst Curie, Unite Genet Mol Levures, CNRS,URA 2171, F-75724 Paris 15, France

[2] Univ Paris 06, Inst Curie, UFR 927, F-75724 Paris, France

来源：

NUCLEIC ACIDS RESEARCH | 2002年 / 30卷 / 16期

关键词：

D O I：

10.1093/nar/gkf483

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Trinucleotide repeats are involved in several neurological disorders in humans. DNA sequences containing CAG/CTG repeats are prone to slippage during replication and double-strand break repair. The effects of trinucleotide repeats on transcription and on nuclear export were analyzed in vivo in yeast. Transcription of a CAG/CTG trinucleotide repeat in the 3'-untranslated region of a URA3 reporter gene leads to transcription of messenger RNAs several kilobases longer than the expected size. These long mRNAs form more readily when CAG rather than CTG repeats are transcribed. CAG- or CUG-containing transcripts show a non-homogeneous cellular localization. We propose that long mRNAs result from transcription slippage, and discuss the possible implications for human diseases.

引用

页码：3540 / 3547

页数：8

共 41 条

[1] Amino acid reiterations in yeast are overrepresented in particular classes of proteins and show evidence of a slippage-like mutational process [J].