Host adaptation to viruses relies on few genes with different cross-resistance properties

被引:93
作者
Martins, Nelson E. [1 ]
Faria, Vitor G. [1 ]
Nolte, Viola [2 ]
Schoeltterer, Christian [2 ]
Teixeira, Luis [1 ]
Sucena, Elio [1 ,3 ]
Magalhaes, Sara [3 ,4 ]
机构
[1] Inst Gulbenkian Ciencias, P-2780156 Oeiras, Portugal
[2] Univ Vet Med Vienna, Inst Populat Genet, A-1210 Vienna, Austria
[3] Univ Lisbon, Fac Ciencias, Dept Biol Anim, P-1749016 Lisbon, Portugal
[4] Univ Lisbon, Fac Ciencias, Ctr Biol Ambiental, P-1749016 Lisbon, Portugal
基金
奥地利科学基金会; 欧洲研究理事会;
关键词
host-parasite interactions; experimental evolution; Drosophila immunity; evolve and resequence; DROSOPHILA-MELANOGASTER RESISTANCE; LONG-TERM EVOLUTION; TRADE-OFFS; PARASITE INTERACTIONS; COURTSHIP SONG; SIGMA-VIRUS; POPULATIONS; FITNESS; COSTS; TRANSMISSION;
D O I
10.1073/pnas.1400378111
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Host adaptation to one parasite may affect its response to others. However, the genetics of these direct and correlated responses remains poorly studied. The overlap between these responses is instrumental for the understanding of host evolution in multiparasite environments. We determined the genetic and phenotypic changes underlying adaptation of Drosophila melanogaster to Drosophila C virus (DCV). Within 20 generations, flies selected with DCV showed increased survival after DCV infection, but also after cricket paralysis virus (CrPV) and flock house virus (FHV) infection. Whole-genome sequencing identified two regions of significant differentiation among treatments, from which candidate genes were functionally tested with RNAi. Three genes were validated-pastrel, a known DCV-response gene, and two other loci, Ubc-E2H and CG8492. Knockdown of Ubc-E2H and pastrel also led to increased sensitivity to CrPV, whereas knockdown of CG8492 increased susceptibility to FHV infection. Therefore, Drosophila adaptation to DCV relies on few major genes, each with different cross-resistance properties, conferring host resistance to several parasites.
引用
收藏
页码:5938 / 5943
页数:6
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