Morphological, biochemical, and molecular characterization of Meloidogyne spp. populations from Brazilian soybean production regions

被引：0

作者：

de Oliveira, Camilla Martins ^{[1
]}

Souza Junior, Ismail Teodoro ^{[2
]}

de Araujo Filho, Jeronimo Vieira ^{[2
]}

de Freitas, Marcos Augusto ^{[3
]}

da Rocha, Mara Rubia ^{[1
]}

Petrofeza, Silvana ^{[2
]}

机构：

[1] Univ Fed Goias, Lab Nematol, BR-74690900 Goiania, Go, Brazil

[2] Univ Fed Goias, Lab Biol Mol Fungos, Goiania, Go, Brazil

[3] JEM Anal Agr, Aparecida De Goiania, Go, Brazil

来源：

CIENCIA RURAL | 2017年 / 47卷 / 05期

关键词：

root-knot nematode; Glycine max; esterase; molecular identification; INCOGNITA;

D O I：

10.1590/0103-8478cr20160634

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Soybean is a commodity of great economic importance worldwide, particularly in Brazil, world's second largest producer. Nematodes, especially those of the Meloidogyne genus, severely limit productivity. Identification of nematode species is important for effective soybean management. Here, 26 populations of root-knot nematode (Meloidogyne spp.) from 15 municipalities in the states of Bahia, Mato Grosso, Goias, and Minas Gerais were characterized based on the morphology of the female perineal region, esterase profile, and identification based on amplification of specific regions of the population genome. Among the Meloidogyne spp. populations obtained, M. incognita and M. javanica, were identified. No mixed populations were present in the samples. Diagnosis based on molecular analysis was shown to be reliable and the fastest for characterization of nematode populations compared to other methods analyzed.

引用

页数：4

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