Time-domain fluorescence lifetime imaging applied to biological tissue

被引：137

作者：

Elson, D ^{[1
]}

Requejo-Isidro, J ^{[1
]}

Munro, I ^{[1
]}

Reavell, F ^{[1
]}

Siegel, J ^{[1
]}

Suhling, K ^{[1
]}

Tadrous, P ^{[1
]}

Benninger, R ^{[1
]}

Lanigan, P ^{[1
]}

McGinty, J ^{[1
]}

Talbot, C ^{[1
]}

Treanor, B ^{[1
]}

Webb, S ^{[1
]}

Sandison, A ^{[1
]}

Wallace, A ^{[1
]}

Davis, D ^{[1
]}

Lever, J ^{[1
]}

Neil, M ^{[1
]}

Phillips, D ^{[1
]}

Stamp, G ^{[1
]}

French, P ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Phys, Photon Grp, London SW7 2AZ, England

来源：

PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES | 2004年 / 3卷 / 08期

关键词：

D O I：

10.1039/b316456j

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Fluorescence lifetime imaging (FLIM) is a functional imaging methodology that can provide information, not only concerning the localisation of specific fluorophores, but also about the local fluorophore environment. It may be implemented in scanning confocal or multi-photon microscopes, or in wide-field microscopes and endoscopes. When applied to tissue autofluorescence, it reveals intrinsic excellent contrast between different types and states of tissue. This article aims to review our recent progress in developing time-domain FLIM technology for microscopy and endoscopy and applying it to biological tissue.

引用

页码：795 / 801

页数：7

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