Regenerated cellulose scaffolds: Preparation, characterization and toxicological evaluation

被引：29

作者：

de Araujo Junior, Adalberto M. ^{[1
]}

Braido, Guilherme ^{[2
]}

Saska, Sybele ^{[1
]}

Barud, Hernane S. ^{[1
,3
]}

Franchi, Leonardo P. ^{[4
]}

Assuncao, Rosana M. N. ^{[5
]}

Scarel-Caminaga, Raquel M. ^{[2
]}

Capote, Ticiana S. O. ^{[2
]}

Messaddeq, Younes ^{[1
]}

Ribeiro, Sidney J. L. ^{[1
]}

机构：

[1] UNESP, Sao Paulo State Univ, Inst Chem, BR-14800970 Araraquara, SP, Brazil

[2] UNESP, Sao Paulo State Univ, Dent Sch Araraquara, BR-14803901 Araraquara, SP, Brazil

[3] Ctr Univ Araraquara UNIARA, Lab Quim Med & Med Regenerat QUIMMERA, Araraquara, SP, Brazil

[4] Univ Sao Paulo, USP, Ribeirao Preto Med Sch, BR-3900 Ribeirao Preto, SP, Brazil

[5] Univ Fed Uberlandia, Fac Ciencias Integradas Pontal, BR-38304402 Ituiutaba, MG, Brazil

来源：

CARBOHYDRATE POLYMERS | 2016年 / 136卷

基金：

巴西圣保罗研究基金会;

关键词：

Regenerated cellulose; Scaffold; Tissue repair; Cytotoxicity; Genotoxicity; Mutagenicity; DNA-DAMAGE; IN-VITRO; BACTERIAL CELLULOSE; REPAIR; BIODEGRADABILITY; BIOCOMPATIBILITY; DEGRADATION; LYMPHOCYTES; BEHAVIOR; ASSAY;

D O I：

10.1016/j.carbpol.2015.09.066

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Regenerated cellulose scaffolds (RCS) may be used as alloplastic materials for tissue repair. In this work, the RCS were obtained by viscose process and characterized by scanning electron microscopy (SEM), wide angle X-ray diffraction (WAXD), Fourier transform infrared spectroscopy (FTIR) and thermogravimetry analysis (TG). In vitro enzymatic degradation assay and toxicological assays were also evaluated. The physicochemical characterizations revealed the formation of a porous material with distinct thermal profile and crystallinity compared to pristine cellulose pulp. Enzymatic degradation assay revealed that lysozyme showed a mildest catalytic action when compared to cellulase, Tricoderma reesei (Tr). Nevertheless, both enzymes were efficient for degrading the RCS. RCS did not show cytotoxicity, mutagenic or genotoxic effects. The systematically characterization of this work suggests that RCS presented distinct features that make it a viable material for future studies related to the development of scaffolds for biological applications. (C) 2015 Elsevier Ltd. All rights reserved.

引用

页码：892 / 898

页数：7

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